| HPLC法测定不同产地硫熏和烘干金银花中绿原酸含量 |
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| 引用本文： | 邢志霞,崔燕兵,刘伟.HPLC法测定不同产地硫熏和烘干金银花中绿原酸含量[J].中国医药导报,2013,10(6):98-99. |
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| 作者姓名： | 邢志霞 崔燕兵 刘伟 |
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| 作者单位： | [1]漯河医学高等专科学校．河南漯河462002 [2]河南中医学院分析测试中心,河南郑州450008 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目（项目编号：2010A360016）. |
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| 摘 要： | 目的比较不同产地硫熏和烘干金银花中绿原酸含量变化，考察硫熏对金银花内在化学成分的影响。方法采用HPLC法测定绿原酸的含量，Cts柱（250mm×4．6mm，5μm），流动相：O．4％磷酸溶液-乙腈=87：13，流速为1．0mL／min；柱温为30℃；检测波长为327nm。结果绿原酸在0．0836-0．4180斗g范围内呈良好线性关系，r=0．9999，平均回收率99．30％（RSD=0．49％）。结论硫熏后金银花绿原酸含量明显增加，不同产地之间的含量差异也较大。
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| 关 键 词： | 高效液相法 硫熏 烘干 金银花 绿原酸 含量测定 |
Determination of the content of Chlorogenic acid in sulfur fumigation and drying honeysuckle of different areas by HPLC |
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| XING Zhixia,CUI Yanbing,LIU Wei.Determination of the content of Chlorogenic acid in sulfur fumigation and drying honeysuckle of different areas by HPLC[J].China Medical Herald,2013,10(6):98-99. |
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| Authors: | XING Zhixia CUI Yanbing LIU Wei |
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| Institution: | 1.Luohe Medical College, He＇nan Province, Luohe 462002, China; 2.Center of Analysis and Measurement, He＇nan College of TCM, He＇nan Province, Zhengzhou 450008, China |
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| Abstract: | Objective To determine the Chlorogenic acid content in sulfur fumigation and drying honeysuckle with HPLC, in order to investigate methods of sulfur fumigation process quality status under the honeysuckle. Methods HPLC, C18 （250 mm × 4.6 mm, 5 μm） was used; with a mobile phase of 0.4%H3PO4- Acetonitrile （87：13）; the flow rate was 1.0 mL/min; the column temperature was 30℃; the detective wavelength was 327 nm. Results The linear range of Chlorogenic acid was from 0.083 6-0.418 0 μg, r = 0.999 9, the average recovery was 99.30% （RSD = 0.49%）. Conclusion The content of Chlorogenic acid has increased significantly after smoked by sulfur, with large differences between different areas. |
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| Keywords: | HPLC Sulfur Drying Honeysuckle Chlorogenic acid Content determination |
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