α—平滑肌肌动蛋白在瘢痕成纤维细胞中的表达

目的 研究转化生长因子-β1(TGF-β1)对瘢痕成纤维细胞中α-平滑肌肌动蛋白（α-SMA）表达的诱导作用。方法 以5例增生性瘢痕为实验组，3例正常瘢痕为对照组，采用成纤维细胞二维培养和三维培养体系及LSAB免疫组织化学染色技术，观察在不同TGF-β1浓度作用下，来源于实验组和对照组的成纤维细胞表达α-SMA的情况。结果 实验组的成纤维细胞，三维培养体系中α-SMA含量明显低于二维培养体系，不同浓度TGF-β1诱导瘢痕成纤维细胞表达α-SMA的作用不同，以5ng/ml的TGF-β1作用最有效；与对照组的成纤维细胞相比，实验组的成纤维细胞在表达α-SMA方面，对TGF-β1的反应更为敏感。结论 在体外，TGF-β1诱导α-SMA在瘢痕成纤维细胞中的表达作用存在着浓度差异；实验组与对照组中的成纤维细胞在细胞性状方面存在差异，对TGF-β1敏感性不同；细胞外基质成分可能会降低TGF-β1的诱导作用，使α-SMA的表达减少。

EXPRESSION OF

OBJECTIVE: To explore the expression of alpha-smooth muscle actin (alpha-SMA) induced by transforming growth factor beta 1 (TGF-beta 1). METHODS: Five samples of hypertrophic scars and three samples of normal mature scars were collected as the experimental and control groups respectively. The fibroblasts were isolated from scars, and cultured in 2-dimension or 3-dimension culture system. The immunohistochemical staining method of LSAB were used to investigate the expression of alpha-SMA in fibroblasts in the different concentration of TGF-beta 1. RESULTS: The expression of alpha-SMA in 3-dimension culture system were markedly lower than those in 2-dimension culture system with respect to the fibroblasts in the experimental group. The expression of alpha-SMA in fibroblasts were different in response to various TGF-beta 1 concentration, it was more effective at the concentration of 5 ng/ml. The expression of alpha-SMA in the fibroblasts from hypertrophic scars seemed to be more sensitive to TGF-beta 1 compared to that of the normal mature scars. CONCLUSION: There are concentration-dependent in the expression of alpha-SMA induced by TGF-beta 1 in scar fibroblasts in vitro. The biological characteristics of the fibroblasts from hypertrophic scars and normal mature scars and their sensitivity to the inducement of TGF-beta 1 were different. The inducement of TGF-beta 1 may be depressed by extracellular matrix components and that may decrease the expression of alpha-SMA.