益骨胶囊含药血清对SD大鼠成骨细胞分泌NO、NOS的影响

目的:观察益骨胶囊含药血清对体外培养的大鼠成骨细胞(OB)表达NO、NOS的影响.方法:(1)将40只12月龄SD大鼠分为益骨胶囊组(高、中、低剂量组)和生理盐水对照组,制备含药血清和对照血清;确定最佳灌胃剂量组和最佳血清添加浓度.(2)分离、培养新生SD大鼠的颅骨成骨细胞,传至第3代后分为2组(含药血清组和空白血清对照组),分别在培养24 h、48 h和72 h后用RT-PCR和试剂盒检测OB表达NOSmRNA的量及其活性变化和OB分泌NO的量.结果:益骨胶囊含药血清组在24h、48 h和72 h时OB表达NOSmRNA的量及其活性和分泌NO的量均明显高于空白血清对照组(P＜0.01或P＜0.05).结论:益骨胶囊含药血清能促进OB NO的分泌和NOS的表达,提示这可能是该方防治骨质疏松的机理之一.

Effect of Yigu Capsule on NO and NOS of Rat Osteoblasts in Vitro

Objective:The effects of Yigu Capsule on NO and NOS of osteoblasts were studied.Methods:(1)Forty 12-month old Sprague-daweley female rats were divided into randomly four groups (Yigu Capsule high dose group,medium dose group and low dose group;saline group),the drug-containing serum and control serum were prepared.(2)The new-born Sprague-daweley rat Osteoblasts were cultured with different Yigu Capsule drug-containing serum concentration and at different exposure time.The mRNA levels of eNOS and iNOS were measured by RT-PCR;the activities of eNOS and iNOS were measured by test kit at different exposure time.NO secretion of osteoblasts was observed,too.Results:The mRNA levels and the activities of eNOS and iNOS and NO secretion of osteoblasts were higher than the control groups respectively after 48,72 and 96 hours (P<0.01 or P<0.05). Conclusions: Yigu Capsule drug-containing serum can promote NOS mRNA and NOS activities expressions and NO secretion of osteoblasts.These results may be parts of the mechanism of Yigu Capsule preventing and treating osteoporosis.