三氧化二砷联用华蟾素对K562细胞增殖和凋亡的影响

目的 研究三氧化二砷联用华蟾素对K562细胞增殖和凋亡的影响,为三氧化二砷和华蟾素联合应用于临床提供理论依据.方法 细胞增殖采用细胞生长及活力测定;细胞凋亡采用细胞形态、Annexin-V/PI双染实验、DNA的PI染色及DNA电泳等方法 测定.结果 在三氧化二砷和华蟾素作用下K562细胞生长受抑伴随活力下降,1.0 μmol/L As2O3、0.125μg/rnl华蟾素、0.25 μg/ml华蟾素、1.0 μmol/L As2O3＋0.125μg/ml华蟾素、1.0 μmol/L As2O3＋0.25μg/ml华蟾素作用K562细胞24 h和48 h后,增殖抑制率分别为（24±1.3）%、（21±1.5）%、（38±3.1）%、（57±2.7）%、（66±3.3）%及（49±2.9）%、（48±2.7）%、（61±2.1）%、（77±3.8）%、（82±4.2）%,细胞凋亡率分别为（4.8±0.5）%、（5.6±0.7）%、（9.8±0.6）%、（11.9±1.2）%、（15.2±1.5）%及（11.0±0.9）%、（12.9±1.1）%、（18.4±1.5）%、（21.0±2.0）%、（28.0±1.9）%,凋亡细胞百分率呈时间剂量依赖关系;基因组DNA电泳出现＂梯＂状条带.结论 三氧化二砷和华蟾素均有抑制K562细胞增殖和诱导该细胞凋亡的作用,两药联用效果更佳.

Effects of As2 O3 in combination with cinobufacini on proliferation and apoptosis of the K562 cells

Objective To investigate the effect of As2 O3 in combination with cinobufacini on proliferation and apoptosis of the K562 cells and provide theoretical basis for clinical application.Methods Cell proliferation was assayed by analyzing the growth and viability of the cells.Apoptosis was assayed by performing cell morphology,Annexin-V/PI staining,DNA-PI staining,and DNA gel electrophoresis.Results After exposure to As2O3 and cinobufacini,the growth of K562 cells was inhibited and the viability of K562 cells was decreased. After treated with 1.0μmol/L As2O3,0.125μg/ml cinobufacini,0.25μg/ml cinobufacini,1.0μmol/L As2O3 + 0.125 μg/ml cinobufacini,1.0μmol/L As2O3 + 0.25μg/ml cinobufacini for 24 and 48 hours,the proliferation inhibition rate were(24 ± 1.3)%,(21 ± 1.5)%,(38 ± 3.1)%,(57 ±2.7)%,(66 ±3.3)% and(49 ±2.9)%,(48 ±2.7)%,(61 ±2.1)%,(77 ±3.8)%,(82 ±4.2)%,the apoptosis rate of K562 cells were(4.8 ± 0.5)%,(5.6 ± 0.7)%,(9.8 ± 0.6)%,(11.9 ± 1.2)%,(15.2±1.5)% and(11.0 ±0.9)%,(12.9 ±1.1)%,(18.4 ±1.5)%,(21.0 ±2.0)%,(28.0 ±1.9)%.The percentage of apoptotic cells was a time- and dose-dependent manner.Typical DNA ladder was shown by DNA gel electrophoresis.Conclusions As2O3 combined with cinobufacini inhibited the proliferation of K562 cells and induced apoptosis of the K562 cells,the combination of the two drugs had better effect.