HPLC法同时测定白英叶中绿原酸、咖啡酸及芦丁的含量

目的:建立HPLC法同时测定白英叶中绿原酸、咖啡酸及芦丁含量的方法。方法:采用HPLC法,Angilent ZorbaxODS C₁₈色谱柱（250 mm×4.6 mm,5μm）,柱前加保护柱;流动相为乙腈-1%冰醋酸溶液,梯度洗脱;流速:0.6 ml·min^-1;柱温:25℃;检测波长327 nm。结果:绿原酸质量浓度在2.16～43.20μg.·ml^-1（r=0.999 5）内与峰面积呈良好的线性关系,平均回收率为99.05%,RSD=1.29%;咖啡酸质量浓度在2.15～43.04μg·ml^-1（r=0.999 8）内与峰面积呈良好的线性关系,平均回收率为100.04%,RSD=1.65%;芦丁质量浓度在5.24～104.74μg·ml^-1内与峰面积呈良好的线性关系,平均回收率99.60%,RSD=1.74%。结论:该方法准确、重复性良好,能同时测定白英叶中的3种有效成分,可用于白英药材及饮片的质量控制。

Simultaneous Determination of Chlorogenic acid,Caffeic acid and Rutin in Bittersweet Leaves by HPLC

Objective： To establish an HPLC method for the determination of chlorogenic acid,caffeic acid and rutin in Bittersweet leaves. Method： The Agilent Zorbax ODS Cls columm （250 mm ×4. 6 mm,5 μm） was used. The detection wavelength was 327 nm. The mobile phase consisted of acetonitrile-1% acetic acid with flow rate of 0. 6 ml·min^-1. The column temperature was 25 ℃. Result： The linear range of chlorogenic acid, caffeic acid and rutin was 2. 16-43.21μg·ml^-1 （ r = 0. 999 5 ） ,2. 15-43.04 μg·ml^-1（ r = 0. 999 8 ） and 5.24-104. 74 μg·ml^-1 （ r = 0. 999 2 ）, respectively. The average recovery was 99. 05% （ RSD = 1.29% ）, 100.04% （ RSD = 1.65% ） and 99. 60% （RSD = 1.74% ） ,respectively. Conclusion： The method is accurate and suitable for the determination of chlorogenic acid, caffeic acid and rutin in bittersweet leaves to control the quantity.