| LC-MS/MS法测定人血浆中氨溴索浓度及其生物等效性研究 |
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| 引用本文： | 刘博,彭可垄. LC-MS/MS法测定人血浆中氨溴索浓度及其生物等效性研究[J]. 中国药师, 2011, 14(3): 349-352 |
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| 作者姓名： | 刘博 彭可垄 |
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| 作者单位： | 1. 华中科技大学同济医学院第二临床医学院,武汉,430030
2. 华中科技大学同济医学院药学院 |
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| 摘 要： | 目的:建立LC-MS/MS法测定人血浆中氨溴索浓度,并进行生物等效性研究。方法:血浆样品用乙腈沉淀蛋白,内标为卡马西平,采用电喷雾化离子源,监测的氨溴索和内标的离子对分别为m/z379.0/263.9和237.0/194.1,色谱柱为UltimateXB-CN（2.1 mm×150 mm,5μm）,流动相为乙腈:0.2%甲酸=40:60,流速为0.3 ml·min-1,进样量为10μl。结果:氨溴索标准曲线线性范围为0.53～187.50 ng·ml-1,最低定量限为0.53 ng·ml-1,低、中、高三种浓度的批内和批间精密度RSD（%）均小于15%,准确度分别为101.41%、105.40%和106.04%,提取回收率分别为82.51%、93.57%和89.19%,基质效应分别为90.48%、92.36%和100.81%,稳定性试验表明样品在本试验条件下稳定。结论:该方法灵敏度高、专属性强、准确、简便,适用于氨溴索的生物等效性研究。
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| 关 键 词： | 氨溴索 LC-MS/MS 生物等效性 |
Determination of Ambroxol in Human Plasma by LC-MS/MS: Application in a Bioequivalence Study |
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| Liu Bo,Peng Kelong. Determination of Ambroxol in Human Plasma by LC-MS/MS: Application in a Bioequivalence Study[J]. China Pharmacist, 2011, 14(3): 349-352 |
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| Authors: | Liu Bo Peng Kelong |
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| Affiliation: | Liu Bo~1,Peng Kelong~2(1.The Second Clinical Medical College,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China,2.School of Pharmacy,Tongji Medical College of Huazhong University of Science and Technology) |
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| Abstract: | Objective:To develop an ultra performance liquid chromatography-tandem mass spectrometry method(LC-MS/MS) for the determination of ambroxol in human plasma and its bioequivalence study.Method:Quantitation was performed on a triple quadruple mass spectrometer employing electrospray ionization.The ion pairs for ambroxol and internal standard were monitored at m/z 379.0/263.9 and 237.0/194.1 in positive ion mode,respectively.Carbamazepine was used as the internal standard,and acetonitrile was used to precipita... |
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| Keywords: | LC-MS/MS |
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