枳椇子的提取工艺及含量测定

目的:优选枳棋子的最佳提取工艺,测定枳椇子中槲皮素的含量。方法:提取工艺研究采用正交试验法,筛选出枳棋子药材的最佳提取工艺。采用高效液相色谱法测定枳棋子有效成分槲皮素的含量。色谱柱:ODS（250 mm×4.6 mm,5μm）;流动相-乙腈-水（40:60）;波长:360 nm;流速:1.0 ml·min^-1;柱温:室温。结果:槲皮素的最佳提取工艺为:药材加入12倍量的50%乙醇溶液提取1.5 h。槲皮素浓度在2.5～50μg·ml^-1之间与峰面积线性关系良好;平均加样回收率为101.1%,RSD=1.7%。结论:本法简单,专属性强,重复性好。

Study on Extracting Process and Content Determination of Hovenia dulcis Thunb.

Objective： To optimize the extracting condition of quercetin in Hovenia dulcis Thunb. , and to determine the content. Method ： The extracting process was optimized by orthogonal design. The content of quercetin in Hovenia dulcis Thunb was measured by HPLC. The conditions were as follows：the chromatographic column was Platisil ODS （250 mm ×4.6mm,5μm） with detection wavelength 360 nm, the mobile phase was acetonitrile-water （40： 60） with a flow rate 1.0 ml· min^-1at room-temperature. Result： The optimal extracting condition was the following： the medical materials were recirculated with 50% alcohol which was 12 times of the medical materials for 1.5 h. The liner range of quercetin was 2.5-50μg·ml^-1. The average recovery was 101.1% with RSD of 1.7%. Conclusion： The studies show that these methods are sufficiently simple, sensitive and reproducible.