紫杉醇对肝癌SMMC7721细胞凋亡的诱导作用及对相关基因表达的影响

目的：探讨紫杉醇对肝癌SMMC7721细胞凋亡的诱导作用及对bcl-2和bax基因的影响。方法：用浓度为5ug·ml-1的紫杉醇处理肝癌SMMC7721细胞后，利用DNA琼脂糖凝胶电泳，TUNEL法检测其细胞凋亡；通过原位杂交法、免疫组化法，测定紫杉醇诱导肝癌SMMC7721细胞产生凋亡时，凋亡抑制基因bcl-2和凋亡促进基因bax的mRNA和蛋白表达。结果：紫杉醇作用一定时间后，SMMC7721细胞产生凋亡、bcl-2显著下降、bax表达显著增高。结论：紫杉醇通过调节细胞凋亡抑制基因bcl-2和细胞凋亡促进基因bax的作用，从而诱导肝癌SMMC7721细胞产生凋亡。

Induction of Taxinol in the Apoptosis of Human Hepotoma Cell Line SMMC7721 and the Effect on the Expressions of Apoptosis Associated Genes

Objective： To explore the apoptotic induction of taxinol and its effect on apoptosis-associated genes in SMMC7721 cell line, Method： The cells were cultured in DMED medium and treated with taxinol in the concentration of 5 ug.ml-1. The apoptosis in the cells was detected by the method of DNA agarose gel with terminal deoxynucl neotidyl transferase mediated dUTP nick end labelling （TUNEL）. The expressions of bcl-2 and bax genes in SMMC7721 cell line were detected by in situ hybridization and immunohistochemical methods. Result： Treatment by taxinol could induce the cells apoptosis, bcl-2 gene lower expression, and bax gene higher expression in the cells. Conclusion： Taxinol can regulate the actions of apoptosis-inhibited gene bcl-2 and apoptosis-promoted gene bax to induce apoptosis in SMMC7721 cells.