| HPLC法测定不同来源补骨脂中补骨脂素和异补骨脂素的含量 |
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| 引用本文： | 唐丽琴,张善堂,陈象青,卢今,董小平.HPLC法测定不同来源补骨脂中补骨脂素和异补骨脂素的含量[J].中国药师,2011,14(3):377-378. |
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| 作者姓名： | 唐丽琴 张善堂 陈象青 卢今 董小平 |
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| 作者单位： | 安徽医科大学附属省立医院药剂科,合肥,230001 |
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| 摘 要： | 目的：建立HPLC法测定不同来源补骨脂中补骨脂素和异补骨脂素含量的方法。方法：选用Hypersil C18柱（250mm×4．6mm，5μm），以甲醇：0．015％磷酸溶液（40：60）为流动相，流速1．0ml·min^-1，检测波长246／1／11，柱温25℃。结果：补骨脂素在4．16～14．56μg·ml^-1间呈良好的线性关系（r=0．9997），平均加样回收率为99．9％（RSD=0．90％，n=9）；异补骨脂素在4．16～14．56μg·ml^-1间呈良好的线性关系（r=0．9996），平均加样回收率为98．0％（RSD=0．78％，n=9）。结论：该方法快速、准确，可用于补骨脂的质量控制。
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| 关 键 词： | HPLC法 补骨脂 补骨脂素 异补骨脂素 |
Determination of Psoralen and Isopsoralen in Psoralea from Different Sources by HPLC |
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| Tang Liqin,Zhang Shantang,Chen Xiangqin,Lu Jin,Dong Xiaoping.Determination of Psoralen and Isopsoralen in Psoralea from Different Sources by HPLC[J].China Pharmacist,2011,14(3):377-378. |
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| Authors: | Tang Liqin Zhang Shantang Chen Xiangqin Lu Jin Dong Xiaoping |
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| Institution: | Tang Liqin,Zhang Shantang,Chen Xiangqin,Lu Jin,Dong Xiaoping(Department of Pharmacy,Affiliated Anhui Provincial Hospital,Anhui Medical University,Hefei 230001,China) |
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| Abstract: | Objective： To establish an HPLC method for the simultaneous determination of psoralen and isopsoralen in psoralea. Method： The analysis was performed on a column of Hypersil C18（250 mm ×4. 6 mm ,5 μm）. The mobile phase consisted of methanol and 0. 015% H3PO4 （40： 60） with the flow rate of 1.0 ml·min^-1. The detection wavelength was 246 nm and the column temperature was 25℃. Result： Promising linearity was obtained within the range of 4. 16-14. 56 μg·ml^-1 for psoralen and isopsoralen. The average recovery of psoralen and isopsoralen was 99. 9% （RSD = 0. 90%, n = 9） and 98.0% （ RSD = 0. 78% , n = 9 ）, respectively. Conclusion： This method is rapid and accurate and can be used to control the quality of psoralea. |
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| Keywords: | HPLC Psoralea Psoralen Isopsoralen |
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