急性白血病患者p53和MGMT基因突变检测

目的：研究p53和MGMT基因突变在急性白血病发病中的意衣以及p53基因突变与MGMT基因突变的关系。方法：应用聚合酶链反应－单莲构象多态性（PCR－SSCP）技术，对36例急性非淋巴细胞白血病（ANLL），26例急性淋巴细胞白血病（ALL）和23例健康对照组骨髓细胞的p53和MGMT基因突变进行了分析。结果：p53基因突变率在ANLL中为16．7％、ALL中为19．2％，MGMT基因突变率在ANLL中为13．9％、ALL中为15．4％；62例急性白血病中有4例为p53和MGMT2个基因同时发生突变；正常对照组均未发生p53和MGMT基因突变。结论：p53和MGMT基因突变在急性白血病发生与发展中起到一定作用，急性白血病中p53基因突变和MGMT基因突变密切相关。

Detection of p53 and MGMT gene mutations in patients with acute leukemia

Aim: To understand the correlation between p53, MGMT gene mutations and acute leukemia, and the correlation between p53 gene mutations and MGMT gene inactivation. Methods: The bone marrow cells of 36 patients with acute non lymphocytic leukemia (ANLL), 26 patients with acute lymphocytic leukemia (ALL) and 23 cases of normal control were detected for mutations in p53 and MGMT gene using polymerase chain reaction single strand conformation polymorphism ( PCR SSCP ). Results: 16.7% of ANLL and 19.2% of ALL contained p53 gene mutations, 13.9% of ANLL and 15.4% of ALL contained MGMT gene mutations, 4 patients in 62 cases of ALL contained both p53 and MGMT gene mutations simultaneously, and neither p53 nor MGMT gene mutation was found in normal control. Conclusion: p53 and MGMT gene mutations may play an important role in the pathogenesis of acute leukemia, and p53 gene mutations were closely correlated with MGMT gene mutations.