| 甘肃省HIV-1毒株基因序列测定和亚型分析 |
| |
| 引用本文： | 王炳涛,邢辉,于爱玲,邱照铸,石林,魏民,席沧海,马鹏飞,赵煜君,邵一鸣.甘肃省HIV-1毒株基因序列测定和亚型分析[J].中国艾滋病性病,2005,11(5):348-349,375. |
| |
| 作者姓名： | 王炳涛 邢辉 于爱玲 邱照铸 石林 魏民 席沧海 马鹏飞 赵煜君 邵一鸣 |
| |
| 作者单位： | 1. 甘肃省疾病预防控制中心,兰州,730000
2. 中国疾病预防控制中心性病艾滋病预防控制中心,北京,100050 |
| |
| 摘 要： | 目的对甘肃省流行的艾滋病病毒（HIV）开展分子流行病学调查.方法用套式聚合酶链反应对23份甘肃省HIV-1感染者外周血淋巴细胞中前病毒脱氧核糖核酸（DNA）的膜蛋白基因进行扩增,并对C2-V3及其邻区300个核苷酸序列进行测定和分析.结果甘肃省流行的HIV-1分属B＇亚型（6例）、C亚型（1例）、CRF-BC亚型（16例）.其中CRF-BC亚型（70%）为主要流行毒株.结论甘肃省HIV-1流行亚型以重组毒株CRF-BC为主,在吸毒人群中广为流行;加强对高危人群监控,是遏制艾滋病在甘肃省蔓延的重中之重.
|
| 关 键 词： | 艾滋病病毒1型 亚型 序列测定 基因离散率 |
| 文章编号： | 1672-5662（2005）05-0348-03 |
| 收稿时间： | 2004-10-12 |
| 修稿时间： | 2004-10-122004-12-07 |
Subtype and sequence analysis on HIV-1 strains circulating in Gansu province |
| |
| WANG Bing-tao,XING Hui,YU Ai-ling,et al..Subtype and sequence analysis on HIV-1 strains circulating in Gansu province[J].Chinese JOurnal of Aids & STD,2005,11(5):348-349,375. |
| |
| Authors: | WANG Bing-tao XING Hui YU Ai-ling |
| |
| Institution: | Gansu Provincial Center for Disease Control and Prevention , Lanzhou 730000, China |
| |
| Abstract: | Objective To identify the HIV-1 subtype distribution in Gansu province by nucleotide sequencing.Methods 23 DNA fragments of HIV-1 env gene were amplified by nested PCR from PBMC extracted from different subjects infected with HIV-1.The sequences of the env C_2-V_3 region were determined.Results There were at least 3 subtypes circulating in Gansu Province:subtype C(n=1),B'(n=6),and CRF-BC(n=16).The circulating recombinant form(CRF),CRF-BC was the predominant strain.Conclusion The rapid spreading of HIV CRF-BC among injecting drug users(IDU) may have important implications for HIV transmission as well as future intervention design. |
| |
| Keywords: | HIV-1 Subtype Sequence analysis Genetic distance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
