Increased expression of the intercellular adhesion molecule-1 (ICAM-1) on peripheral blood neutrophils in acute pancreatitis |
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| Affiliation: | 1. Department of Gastroenterology and Internal Medicine, Medical University of Bialystok, Poland;2. Department of Hematological Diagnostics, Medical University of Bialystok, Poland;3. Department of Anesthesiology and Intensive Therapy, Medical University of Bialystok, Poland;1. Department of Paediatric Neurology, Medical University in Lublin, Lublin, Poland;2. Department of Neurology, Medical University of Bialystok, Bialystok, Poland;3. Department of Neurology, Medical University in Lublin, Lublin, Poland;4. Medical Department, Bayer sp. z o.o., Warszawa, Poland;1. Department of Surgery, University Erlangen, Erlangen, Germany;2. Department of Experimental Surgery and Molecular Oncology of Solid Tumors, Medical Faculty Mannheim, University Heidelberg and DKFZ Heidelberg, Heidelberg, Germany;3. Cancer Registry, Department of Surgery, University Erlangen, Erlangen, Germany;4. Institute of Pathology, University Erlangen, Erlangen, Germany;5. Department of Pathology, St. Vincentius Hospital, Karlsruhe, Germany;6. Department of Pathology, Klinikum Nord, Nürnberg, Germany;1. Department of Otolaryngology, Jagiellonian University Medical College, Cracow, Poland;2. Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Cracow, Poland;1. Department of Laboratory Diagnostics, Poznan University of Medical Sciences, Poznan, Poland;2. Department of Internal Medicine, Metabolic Disorders and Hypertension, Poznan University of Medical Sciences, Poznan, Poland |
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| Abstract: | PurposeConsidering the important role of neutrophils’ activation in the pathogenesis of acute pancreatitis (AP), the aim of our study was to evaluate the expression of leukocytes’ adhesion molecules in patients with AP.Patients/methodsThirty-five patients (16 women and 19 men; age 32–77 years, median 56 years) with AP were prospectively included into our study. The absolute number of leukocytes was estimated by haematologic analyser. Surface neutrophils antigens (CD) were assayed by the direct fluorescence method for whole blood, using a flow cytometer.ResultsAt the day 1, significant increase of ICAM-1 expression was found in patients with severe AP (S-AP) (7280 mm−3 vs 2850 mm−3 in healthy control; p < 0.05). In the days 2, 3 and 5 it sharply decreased and peaked again to 4860 mm−3 at the day 10. In patients with mild AP (M-AP), not significant elevation of ICAM-1 quickly returned to normal level. In both forms of AP, neutrophil CD62L (L-selectin) expression reached the highest level at the day 1 (8800 mm−3 and 9020 mm−3, respectively in M-AP and S-AP, in comparison to 3400 mm−3 in control; p < 0.05). Expression of CD69 (neutrophils’ marker of early activation) significantly increased in both M-AP and S-AP.ConclusionsWe have found an early and significant increase of peripheral blood neutrophil CD54/ICAM-1 expression, specific for S-AP but not for M-AP. It may provide a good marker predicting severe course of pancreatitis. |
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| Keywords: | Acute pancreatitis Adhesion molecules CD69 Neutrophils |
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