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Evaluation of the Candida albicans removal and mechanical properties of denture acrylics cleaned by a low-cost powered toothbrush
Institution:1. Dental Materials Science, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, P.R. China;2. Oral Sciences, Faculty of Dentistry, National University of Singapore, Singapore, Singapore;1. Professor, Department of Dentistry, University Center of Maranhão, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil;2. Graduate student, Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil;3. Graduate student, Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil;4. Research Fellow, Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil;5. Professor, Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil;6. Professor, Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Piracicaba, Sao Paulo, Brazil;1. Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;2. Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;3. Department of Fixed Prosthodontics, Institute of Health Biosciences Graduate School, The University of Tokushima, Tokushima, Japan;4. Advanced Program in Orofacial Pain and Oral Medicine, Ostrow School of Dentistry, University of Southern California, USA;1. Department of Surgical and Morphological Science, Dental School, University of Varese, Italy;2. Department of Prosthodontic and Implantology, Fondazione IRCCS Ca’ Granda, University of Milan, Italy;3. Department of Oral Rehabilitation, Istituto Stomatologico Italiano, University of Milan, Italy
Abstract:PurposeTo investigate the effects of using a low-cost powered toothbrush for cleaning on dental prostheses made of heat polymerized poly(methyl methacrylate), PMMA.MethodsHeat cured PMMA specimens beam with the dimensions of 45.0 mm × 6.5 mm × 4.5 mm were fabricated. The specimens were kept in water storage at 37 °C constant temperature for 0, 1, 7, 15, 30 and 60 days and randomly assigned for testing or control. Test specimens underwent brushing by using a powered toothbrush at an applied force of 2.00 N for 22 min with water as medium. Surface roughness measurement (Ra), flexural strength and efficacy of brushing to remove coated Candida albicans biofilm were investigated.ResultsThe results of the mean surface roughness value and the flexural strength were analysed by using two-way ANOVA and Tukey post hoc test at 5% significance level. In general, the specimens showed no significant changes in flexural strength after brushing. However, the flexural strength and the surface roughness value were significantly lower in specimens group after 7 days in water storage compared to the control. SEM micrographs of post-brushed specimens revealed satisfactory removal of C. albicans biofilm.ConclusionA low-cost powered toothbrush together with a liquid medium successfully removed C. albicans biofilm on dental acrylic PMMA-based prostheses, without compromising the mechanical properties.
Keywords:Denture  Denture cleaning methods  Poly(methyl methacrylate)  Low-cost powered toothbrush
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