Effects of calcium chelators on intracellular calcium and excitotoxicity

In an attempt to probe the relationship between excitotoxicity and increases in intracellular calcium (Ca²⁺]_i), BAPTA-AM and its analogs were applied to cultured hippocampal neurons. Chelation of Ca²⁺]_i depressed and prolonged transient responses to glutamate and did not effect elevation of Ca²⁺]_i by prolonged exposure. This explains the inability of the chelators to prevent glutamate-induced toxicity.