藻酸钙微载体培养胎儿关节软骨细胞

目的观察胎儿关节软骨细胞在藻酸钙微载体培养中的特征,探讨用藻酸钙微载体培养胎儿关节软骨细胞的优缺点。方法用藻酸钙微载体和体外单层培养法培养胎儿关节软骨细胞,观察细胞形态学变化,测定细胞数量变化及其生长曲线,观察细胞增殖;借助免疫组织化学的方法了解细胞II型胶原的合成情况。结果体外单层培养的胎儿关节软骨细胞传至第5代已出现明显的反分化,多数细胞变为成纤维细胞状,合成II型胶原的能力明显减弱;而用藻酸钙微载体培养的软骨细胞3个月后仍保持有旺盛的合成Ⅱ型胶原的能力。结论用藻酸钙微载体培养胎儿关节软骨细胞可较长时间保持其表型及合成基质能力,防止反分化的发生。

Culture of embryo articular chondrocyte in calcium alginate micro- carrier

Aim To investigate the advantages and disadvantages of calcium alginate on microcarrier culture of fetus articular chondrocytes. Methods The changes of cell morphology,cell quantity and growth curve were investigated among articular chondrocytes cultured by calcium alginate microcarrier to observe the proliferation of chondrocytes. The biologic synthesis of type II collagen in chondrocytes was comprehended by immunohistochemical method.Results The fifth generation of the mono layer culture chondrocytes appeared obvious de differentiation, and most cells became fibroblast like. The cells cultured in alginate micro carrier gels retained the ability to synthesis the type II collagen.Conclusion Culture of embryo articular chondrocytes in calcium alginate carrier gels can retain the cell phono type and ability to synthesize the type II collagen for 3 months, retarding the happening of de differentiation.