| 结直肠癌不同病变阶段K-ras基因突变的研究 |
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| 引用本文： | 沈永奇,叶韵斌,郑雄伟,力超,陈强.结直肠癌不同病变阶段K-ras基因突变的研究[J].肿瘤,2010,30(2). |
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| 作者姓名： | 沈永奇 叶韵斌 郑雄伟 力超 陈强 |
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| 作者单位： | 福建医科大学教学医院,福建省肿瘤医院内科研究室,福州,350014 |
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| 摘 要： | 目的:研究结直肠癌不同病变阶段K-ras基因变化及其对结直肠癌演变的影响.方法:提取20例结直肠癌患者的原发灶、淋巴结转移灶、远处转移灶、结直肠腺瘤和相应正常结直肠组织的DNA各20 份,经PCR扩增,对产物进行基因序列分析.结果: 正常结直肠组织均表达野生型K-ras基因,结直肠腺瘤、原发灶、淋巴结转移灶和远处转移灶的K-ras突变率分别为20.0%(4/20)、30.0%(6/20)、25.0%(5/20)和30.0%(6/20),有3例远处转移灶出现复合突变.在发生K-ras突变的标本中,结直肠腺瘤、淋巴结转移灶、远处转移灶的突变类型与原发灶的一致性分别为0%(0/4)、40.0%(2/5)和50.0%(3/6).结论:结直肠腺瘤、淋巴结转移灶和远处转移灶不宜作为临床检测K-ras突变的常规标本,但在无法获得原发灶标本时,淋巴结转移灶和远处转移灶的检测结果也有一定的参考价值;结直肠癌发生、发展的过程中,K-ras基因可能在多阶段发生多次不同的突变.
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| 关 键 词： | 结直肠肿瘤 基因 ras 突变 DNA突变分析 |
K-ras mutations in colorectal cancer at different stages |
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| SHEN Yong-qi,YE Yun-bin,ZHENG Xiong-wei,LI Chao,CHEN Qiang.K-ras mutations in colorectal cancer at different stages[J].Tumor,2010,30(2). |
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| Authors: | SHEN Yong-qi YE Yun-bin ZHENG Xiong-wei LI Chao CHEN Qiang |
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| Institution: | SHEN Yong-qi,YE Yun-bin,ZHENG Xiong-wei,LI Chao,CHEN Qiang (Internal Oncology Laboratory of Fujian Province,Tumor Hospital of Fujian Medical University,Fuzhou 350014,China) |
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| Abstract: | Objective:To study the alteration of K-ras mutations in different stages of colorectal cancer(CRC) and its influence on the progression of CRC. Methods:The 20 paraffin-embedded tissues,including primary foci,metastatic lymph nodes,remoter metastatic foci,colorectal adenoma,and normal colorectal tissues,were collected from 20 patients with colorectal cancer. The sequence of PCR-amplified products were analyzed. Results:The wild K-ras gene was expressed in normal colorectal tissues. The mutation frequency of ... |
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| Keywords: | Colorectal neoplasms Genes ras Mutation DNA mutational analysis |
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