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| 乳腺癌细胞凋亡、增殖与相关基因表达、突变的关系 | |
| 引用本文： | 李红智,喻林升,王宗敏,谢丽微. 乳腺癌细胞凋亡、增殖与相关基因表达、突变的关系[J]. 中国病理生理杂志, 2003, 19(5): 676-678 |
| 作者姓名： | 李红智 喻林升 王宗敏 谢丽微 |
| 作者单位： | 1. 温州医学院生物学教研室, 浙江 温州 325027; 2. 温州医学院病理学教研室, 浙江 温州 325027; 3. 温州医学院第二附属医院病理科, 浙江 温州 325027 |
| 基金项目： | 浙江省教委科研基金资助项目 ( 98115 5 ) |
| 摘 要： | 目的:研究乳腺癌细胞凋亡、细胞增殖及相关基因表达、突变的关系,为乳腺癌细胞凋亡、细胞增殖失衡的基因调控机制提供依据。方法:分别应用TUNEL法、免疫组化S-P法和PCR-SSCP法检测54例乳腺癌标本凋亡指数(AI)和增殖指数(MI),Bcl-2、p53、c-erbB-2、PCNA、Ki67、TopoⅡ蛋白表达和p53基因突变。结果:54例乳腺癌AI平均9.40±3.78,MI平均5.96±2.36,AI与MI呈显著正相关(r=0.46,P＜0.01)。Bcl-2、PCNA、Ki67、TopoⅡ表达与AI、MI均呈显著正相关(P＜0.01)。p53表达、c-erbB-2表达、p53突变与MI呈显著正相关(P＜0.01)。p53基因突变类型与AI呈显著相关(P＜0.05)。结论:乳腺癌细胞凋亡、增殖失衡与相关基因异常表达、突变有关。 |
| 关 键 词： | 乳腺肿瘤 细胞凋亡 增殖 免疫组织化学 基因表达 |
| 文章编号： | 1000-4718(2003)05-0676-03 |
| 收稿时间： | 2002-03-06 |
Relationship between apoptosis, proliferation and expression, mutation of related genes in breast cancer |
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| LI Hong-zhi ,YU Lin-sheng ,WANG Zong-min ,XIE Li-wei. Relationship between apoptosis, proliferation and expression, mutation of related genes in breast cancer[J]. Chinese Journal of Pathophysiology, 2003, 19(5): 676-678 | |
| Authors: | LI Hong-zhi YU Lin-sheng WANG Zong-min XIE Li-wei |
| Affiliation: | 1. Department of Biology, Wenzhou Medical College, Wenzhou 325027, China; 2. Department of Pathology, Wenzhou Medical College, Wenzhou 325027, China; 3. The Second Affiliated Hospital, Wenzhou Medical College, Wenzhou 325027, China |
| Abstract: | AIM:To study the relationship between apoptosis, proliferation and expression,mutation of related genes in breast cancer.METHODS:Methods of TUNEL, immunohistochemical S-P and PCR-SSCP were used respectively to study apoptotic index (AI), mitotic index(MI), expression of Bcl-2,p53,c-erbB-2,PCNA,Ki67,TopoⅡ and mutation of p53 in 54 cases of breast cancer.RESULTS:AI and MI were 9.40±3.78 and 5.96±2.36, respectively. There was a significant direct correlation between them(r=0.46.P＜0.01).High expression of Bcl-2,PCNA,Ki67,TopoⅡ coincided with high AI,MI(P＜0.01). High expression of p53,c-erbB-2 and mutation of p53 coincided with high MI(P＜0.01). Type of p53 mutation coincided with AI(P＜0.05).CONCLUSION:Disturbance of gene control between apoptosis and proliferation is related with expression,mutation of related genes in breast cancer. |
| Keywords: | Breast neoplasms Apoptosis Proliferation Immunohistochemistry Gene expression |
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