PCNA的表达与人乳腺癌细胞凋亡的关系

目的：研究增殖细胞核抗原（PCNA）的表达与人乳腺癌细胞凋亡的关系。方法：以乳腺癌细胞株MCF-7／S（化疗敏感细胞株）为研究对象，应用MTT比色法检测阿霉素（ADR）对体外培养的MCF-7／S细胞增殖抑制作用，末端标记（TUNEL）法检测ADR诱导乳腺癌细胞凋亡，免疫细胞化学法检测ADR作用前后PCNA的表达。结果：ADR抑制MCF-7／S细胞增殖，旱剂量依赖性，IC50为0．128mg／L；ADR作用组MCF-7／S细胞的凋亡率（Apoptoticrate，AR）为0．261，与对照组细胞的凋亡率0．0449相比明显增高（P〈0．01）；PCNA阳性表达率为0．3371，与对照组PCNA阳性表达率0．5152相比明显降低（P〈0．01）。结论：乳腺癌肿瘤细胞的凋亡率（AR）和PCNA的阳性表达呈负相关；ADR能诱导MCF-7／S细胞凋亡，并能抑制其增殖。

The relationship between PCNA and cell apoptosis in human breast cancer

Objective:To investigate the relationship between proliferating cell nuclear antigen(PCNA) and cell apoptosis in human breast cancer.Methods:MTT colorimetric assay was applied to examine the growth inhibition,and apoptosis rates were determined by terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling(Tunel);The expressive levels of dephosphorylated RB protein and PCNA were detected with immunocytochemistry.Results:MTT assay that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner,the 50% inhibition concentration (IC_50 ) was 0.128mg/L.Tumor cell apoptotic rate(AR) in ADR group(=0.261) was significantly higher than that in control group (=0.0449,P<0.01),PCNA positive expression rate in ADR group (=0.3371) was significantly lower than that in control group (=0.5152,P<0.01).Conclusion:There was significant negative association between AR and PCAN in ADR group and control group;ADR could include MCF-7/S cells apoptosis and inhibit MCF-7/S cells proliferation.