腺病毒介导的HSV—tk体外抗喉癌作用

目的 观察HSV-tk/GCV系统对喉癌细胞的杀伤作用。方法 将带有报告基因LacZ的5型缺陷性腺病毒载体AdCMVLacZ感染喉癌细胞Hep-2,X-gal染色，测定腺病毒的转染率，利用同源重组技术构建含HSV-tk基因的重组腺病毒载体AdCMVtk.AdCMVtk感染Hep-2细胞后，加入10mg/LGCV。观察瘤细胞存活率及旁观者效应。结果 随着腺病毒感染复数量（ultiplicity of infection,MOI)增加，对Hep-2细胞转染率亦增加，100%转染需的MOI为200。AdCMVtk/GCV对Hep-2细胞的杀伤强度与AdCMVtk量呈正向关系。即有浓度依赖性，此杀伤作用存在旁观者效应，但较弱。结论 腺病毒介导的HSV-tk/GCV具有杀伤喉癌细胞作用。

Anti-laryngeal carcinomawith adenovirus vector mediated HSV-tk gene

ObjectiveTo observe killing action of HSV-tk/GCV system on laryngeal cancer cells. Methods Hep-2 cells were transfected by adenovirus vector with LacZ gene, then stained with X-gal for measurement of the adenovirus transfected efficiency. The recombinant adenovirus carrying HSV-tk was constructed by homologous recombination techniques. Hep-2 cells survival and by stander effect were observed after adding 10 mg/L GCV to transfected cells. Results The more the multiplicity of infection (MOI), the more the adenovirus transfected efficiency to Hep-2 cells. 100% transfected efficiency needs MOI 200 The killing action of HSV-tk/GCV on Hep-2 cells depends on the amount of AdCMVtk. There is a weak by stander effect. Conclusion Adenovirus mediated HSV-tk/GCV can kill Hep-2 cells. [