Rapid and sensitive identification of human blood by an ELISA-ABC method using a biotinylated antibody against human HbA0

Summary A direct enzyme-linked immunosorbent assay (ELISA) using an avidin-biotin complex (ABC) system for the identification of human blood is described. In this ELISA-ABC method, in which biotin-labeled goat IgG antibody against human HbA₀ was used, it was possible clearly to distinguish human blood from the blood of other species, including that of Japanese monkeys. It took about 3 h to obtain the results. Human Hb concentrations ranging from 22 ng to 169 g produced a positive reaction, and the minimum detection limit in terms of the highest possible dilution of human blood was 1:640,000.