大鼠部分肝切除后再生肝组织中上调表达基因的克隆与分析

目的克隆肝再生中差异表达的基因,为最终阐明肝再生的调控机制奠定基础。方法采用大鼠短间隔连续肝切除(SISPH)模型,用抑制性消减杂交技术(SSH)研究4～8h之间差异表达的基因。结果用SSH分析0、4h、8h和12h模型的4～8h再生肝,筛选出33个与肝再生有关的上调表达序列标记(EST),其中17个为已在肝再生中报道的已知基因,6个为首次报道的已知基因,10个为大鼠中尚未报道的新基因,并对新基因在NCBI进行注册。结论在肝再生中SSH是一个分离肝再生差异表达基因的强有力工具,这些差异基因的分离,尤其是在肝再生中首次报道的已知基因和新基因的分离,可为进一步阐明肝再生的机制奠定基础。

Cloning and analysis of the up-regulated gene of regenerative liver in rats after partial hepatectomy

Objective To clone the differentially expressed genes in liver regeneration for finally elucidating the regulatory mechanism of liver regeneration.Methods In this study,adopting short interval successive partial hepatectomy (SISPH) as experimental model,and recurring to suppression subtractive hybridization (SSH)technique,we constructed 4 h-8 h of 0-4-8-12 h forward subtracted library.Results Thirty-three differentially up-regulated expression ESTs related to liver regeneration were gained,among which 17 ESTs were related to reported genes of liver regeneration,6 up-regulated ESTs were known but firstly reported in liver regeneration and 10 up-regulated ESTs were rat novel genes.Conclusion These results demonstrate that the SSH technology is a powerful tool in analyzing the differentially expressed genes.And the isolation of the differentially expressed genes have laid foundation to further elucidate the molecular mechanism of liver regeneration.