坐骨神经火器性断裂伤后腰脊髓及脊神经节内微循环损伤特点

目的 :研究坐骨神经火器性断裂伤后脊髓和脊神经节内微循环的变化特点。方法 :通过 5 3式滑膛枪建立兔坐骨神经火器性断裂伤模型 ,在伤后第 1、3、7、15、3 0天取材。采用伊文思兰 (Evan’sblue ,EB)示踪、光电镜观察、组织含水量测定等方法检测脊髓、脊神经节内微循环变化特点。结果 :坐骨神经火器性断裂伤后 ,脊髓及脊神经节内微循环受到破坏 ,表现为内皮细胞变性、坏死及脱落 ,对EB的通透性明显增高 ,组织含水量增高 ,3天达高峰 ,脊神经节损伤较脊髓严重 ;伤后 3 0天腰脊髓、脊神经节内微循环的通透性仍高于正常。结论 :脊神经节及脊髓内微循环受到破坏是坐骨神经火器性断裂伤的重要特征之一 ,脊神经节损伤较脊髓严重

Characteristics of Microcirculatory Changes in Lumbar Spinal Cord and Ganglion After Firearm Sciatic Nerve''''s Disruptive Injury in Rabbits

Objective: To explore the permeability alteration of microcirculation in the firearm sciatic nerve disruptive injury of rabbits.Method: The model of firearm sciatic nerve's disruptive injury in rabbits were established with 53 type musket, and the species were harvested at 1day, 3day, 7day, 15day and 30day post injury respectively. The changes of the microcirculation in lumbar spinal cord and ganglion were analyzed with Evan's blue, light and electron microscope and water content measurement.Results: The microcirculation in the lumbar spinal cord and dorsal root ganglion(DRG) are damaged after the firearm sciatic nerve's disruptive injury. Parts of the endothelial cell are degenerative, necrotic and exfoliative, which leading to the increase of vascular permeability and of water content, with the peak time at 3day post injury. The change in the dorsal root ganglion is more serious than that in the spinal cord, and the abnormal states is kept more than 30 days.Conclusion: Both of the microcirculation in the spinal cord and dorsal root ganglion are severely destroyed after firearm sciatic nerve's disruptive injury, and the degree in dorsal root ganglion is more serious than that in lumar spinal cord.