鸡肠中血管活性肠肽的提取与测定

目的研究鸡肠不同部位血管活性肠肽的含量及其不同提取方法对血管活性肠肽含量的影响。方法分别取新鲜鸡的十二指肠、空肠、和回肠段,除去油脂及污物,用水冲洗干净,甩干水分,分别称取20.0g,采用不同方法提取,用酶联免疫吸附法测定血管活性肠肽的含量。结果鸡的十二指肠、空肠和回肠部位血管活性肠肽含量分别为25.53,25.01和23.27ng/g;热水提取含量较高,在酸性条件下加热容易破坏。结论鸡的全肠均含丰富的血管活性肠肽。

Extraction and determination of vasoactive intestinal peptide in intestine of chicken

Purpose To determinate the vasoactive intestinal peptide in intestine of chicken and to compare the extraction technologies.Methods Duodenum,jejunum,and ileum segment of fresh intestine of chicken were first defatted,and then cleaned with water.ELISA method was used to detect the content of vasoactive intestinal peptides respectively after they were extracted from the processed intestinal segments(20.0 g respectively) by different methods.Results The content of vasoactive intestinal peptide in duodenum,jejunum,and ileum segment was 25.53,25.01 and 23.27 ng/g respectively.The content is higher by the extraction in hot water and vasoactive intestinal peptide is destroyed in acidic and hot environment.Conclusion Vasoactive intestinal peptide is rich in intestine of chicken.