| 零陵香水溶性多糖组分的GC-MS分析 |
| |
| 引用本文： | 刘长松,张贵君,张智圆,李奇豫,董文茜,李景松,张雅楠. 零陵香水溶性多糖组分的GC-MS分析[J]. 中国实验方剂学杂志, 2012, 18(19): 129-131 |
| |
| 作者姓名： | 刘长松 张贵君 张智圆 李奇豫 董文茜 李景松 张雅楠 |
| |
| 作者单位： | 北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102;北京中医药大学中药学院, 北京 100102 |
| |
| 摘 要： | 目的:鉴定零陵香水溶性多糖组分,为建立专属性的质量评价指标和中药药效组分新药研发奠定基础。方法:采用水提醇沉法制备零陵香粗多糖,Sevag法除蛋白、H2O2脱色、透析得到精制多糖,衍生化后用GC-MS分析。结果:零陵香水溶性多糖主要组分依次为鼠李糖醇-阿拉伯糖-核糖-葡萄糖-木糖,其相对含量比例为1.03:9.17:4.74:18.95:17.11。结论:GC-MS联用法可快速鉴定零陵香水溶性多糖组分。
|
| 关 键 词： | 零陵香 水溶性多糖 药效组分 GC-MS 质量评价 |
| 收稿时间： | 2012-03-10 |
Analysis of Water-soluble Polysaccharide Components from Herba Lysimachiae Foenum-graeti by GC-MS |
| |
| LIU Chang-song,ZHANG Gui-jun,ZHANG Zhi-yuan,LI Qi-yu,DONG Wen-xi,LI Jing-song and ZHANG Ya-nan. Analysis of Water-soluble Polysaccharide Components from Herba Lysimachiae Foenum-graeti by GC-MS[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(19): 129-131 |
| |
| Authors: | LIU Chang-song ZHANG Gui-jun ZHANG Zhi-yuan LI Qi-yu DONG Wen-xi LI Jing-song ZHANG Ya-nan |
| |
| Affiliation: | School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;School of Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China |
| |
| Abstract: | Objective:Identifying the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti,to establish the quality of specificity evaluation index and Chinese medicine's active components alignment, which laid the foundation of new drug development. Method:The preparation of polysaccharides was extracted by the methods of water extracting-alcohol precipitating, then Sevag method sinking in protein, H2O2 bleaching and the use of dialysis method refined. The derivative change of the refined polysaccharide was analyzes by GC-MS. Result:The polysaccharide of Herba Lysimachiae Foenum-graeti is composed of rhamnitol, arabinose, ribose, glucose, and xylose, the proportion of it is 1.03:9.17:4.74:18.95:17.11. Conclusion:GC-MS could rapidly ascertain the water-soluble polysaccharide components of Herba Lysimachiae Foenum-graeti. The water-soluble polysaccharide of Herba Lysimachiae Foenum-graeti can be used as a component of the quality evaluation index and drug components of the new drug research based material. |
| |
| Keywords: | Herba Lysimachiae Foenum-graeti water-soluble polysaccharide active components alignment GC-MS quality evaluation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
|
点击此处可从《中国实验方剂学杂志》下载全文 |
|
