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| 排龈时间与药物对牙龈成纤维细胞的毒性比较研究 | |
| 引用本文： | 张晓明,惠敏,刘健. 排龈时间与药物对牙龈成纤维细胞的毒性比较研究[J]. 实用口腔医学杂志, 2011, 27(2): 248-251. DOI: 10.3969/j.issn.1001-3733.2011.02.022 |
| 作者姓名： | 张晓明 惠敏 刘健 |
| 作者单位： | 1. 滨州医学院附属医院口腔修复科,256603 2. 滨州医学院口腔学院 3. 日照市中医院口腔正畸科 |
| 基金项目： | 滨州医学院科技计划(编号:BY2007KJ47) |
| 摘 要： | 目的:研究不同排龈药物与不同排龈时间对人牙龈成纤维细胞(HGF)毒性作用的影响,指导临床选择最佳排龈药物和排龈时间。方法:MTT比色测定细胞活性,透射电镜观察直接损伤后细胞超微结构的变化,排龈药物pH值测定。结果:0.1%盐酸肾上腺素的细胞毒性作用最小,排龈时间5 min细胞毒性小于10 min,硫酸铁的细胞毒性最大。结论:健康患者可采用0.1%盐酸肾上腺素进行排龈;伴有心血管系统疾病的患者,可采用20%硫酸铝替代0.1%盐酸肾上腺素;临床上使用硫酸铁排龈时应适当降低其浓度。 |
| 关 键 词： | 排龈药物 细胞毒性 人牙龈成纤维细胞 |
Toxicity of different retraction agents and exposure time on human gingival fibroblasts in vitro |
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| ZHANG Xiaoming,HUI Min,LIU Jian. Toxicity of different retraction agents and exposure time on human gingival fibroblasts in vitro[J]. Journal of Practical Stomatology, 2011, 27(2): 248-251. DOI: 10.3969/j.issn.1001-3733.2011.02.022 | |
| Authors: | ZHANG Xiaoming HUI Min LIU Jian |
| Affiliation: | ZHANG Xiaoming1,HUI Min2,LIU Jian3.1.256603,Department of prosthodontics,Binzhou medical university affiliated hospital,2.College of stomatology,Binzhou medical university,3.Department of orthodontics,the Hospital of Traditional Chinese Medicine in Rizhao |
| Abstract: | Objective: To choose the best retraction agent and exposure time for the clinic by investigating cytotoxic effects of three chemical retraction agents on human gingival fibroblasts(HGF) in vitro.Methods: The proliferation inhibit was observed by MTT assay,the ultra structure of the cells was observed under transmission electron microscope.Three chemical retraction agents of pH were determinated.Results: 0.1%HCl-epinephrine was the least toxic gingival retraction agent,and cytotoxic effect of 5min treatment ... |
| Keywords: | Chemical retraction agent Cytotoxicity Human gingival fibroblasts |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |