| 人牙髓干细胞向成牙本质细胞定向分化过程中miRNAs表达谱筛选及鉴定 |
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| 引用本文： | 张萍,朱聪惠,张纲,谭颖徽.人牙髓干细胞向成牙本质细胞定向分化过程中miRNAs表达谱筛选及鉴定[J].实用口腔医学杂志,2011,27(2):212-216. |
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| 作者姓名： | 张萍 朱聪惠 张纲 谭颖徽 |
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| 作者单位： | 1. 第三军医大学新桥医院口腔科,重庆,400037
2. 第三军医大学新桥医院肿瘤研究所 |
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| 基金项目： | 国家自然科学基金资助项目(编号:30973334); 重庆市自然科学基金资助项目(编号:CQCTSZ2007BB5016); 第三军医大学科研基金资助项目(编号:2006D136) |
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| 摘 要： | 目的:筛选人牙髓干细胞向成牙本质细胞定向分化过程中差异表达的microRNAs(miRNAs)并进行初步鉴定。方法:体外分离、培养和鉴定人牙髓干细胞,miRNAs芯片筛选牙髓干细胞向成牙本质细胞分化过程中差异表达的miRNAs,并通过TargetScan数据库预测靶基因,实时定量PCR法对结果进行初步鉴定。结果:人牙髓干细胞vimentin、nestin、GFAP和I型胶原4种细胞表型均表达;成脂诱导3周后细胞内有油红O染色阳性脂滴出现;成牙本质诱导可见钙结节形成;基因芯片结果显示,牙髓干细胞向成牙本质细胞分化过程中,发生2倍以上表达变化的miRNAs有6条,其中上调3条,下调3条。通过miRNA靶标预测工具预测靶基因,发现hsa-miR-633和hsa-miR-210有与牙髓干细胞分化相关的靶基因;real time-PCR验证hsa-miR-633和hsa-miR-210表达变化与基因芯片结果相符。结论:人牙髓干细胞经诱导向成牙本质细胞分化过程中miRNAs表达谱具有显著变化,为牙髓干细胞分化机制研究提供了新的提示。
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| 关 键 词： | miRNAs 牙髓干细胞 芯片 定向分化 成牙本质细胞 |
Screening and identification of the expression profile of microRNAs in human dental pulp stem cells during directional differentiation into odontoblasts |
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| ZHANG Ping,ZHU Conghui,ZHANG Gang,TAN Yinghui.Screening and identification of the expression profile of microRNAs in human dental pulp stem cells during directional differentiation into odontoblasts[J].Journal of Practical Stomatology,2011,27(2):212-216. |
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| Authors: | ZHANG Ping ZHU Conghui ZHANG Gang TAN Yinghui |
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| Institution: | ZHANG Ping1,ZHU Conghui2,ZHANG Gang1,TAN Yinghui1.1.400037 Chongqing,Department of Stomatology,Xinqiao Hospital,China,2.Institute of Oncology Research,Third Military Medical University |
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| Abstract: | Objective: To screen and identificate the differential expression microRNAs(miRNAs) of human dental pulp stem cells(DPSCs) during directional differentiation into odontoblasts.Methods: Human DPSCs were isolated,cultrued and identificated in vitro,the differential expression miRNAs of DPSCs were examined during directional differentiation into odontoblasts by miRNAs chip method.The target genes were forecasted by the TargetScan database and identificated by real time-PCR.Results: DPSCs were found to express ... |
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| Keywords: | miRNAs Dental pulp stem cells Gene chip Directional differentiation Odontoblast |
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