间日疟原虫和恶性疟原虫可溶性抗原免疫反应性比较

目的分析和比较间日疟原虫(Plasmodiumvivax,P.v)和恶性疟原虫(P.falciparum,P.f)可溶性抗原与间日疟感染者混合血清和单克隆抗体(McAb)M26-32免疫反应性的差别,以期寻找潜在的疟疾诊断抗原。方法取P.v感染者血样,用Plasmodipur滤器分离去除白细胞,经60%Percoll浓集其中的感染红细胞(i RBC)。分别制备P.v、P.f和正常红细胞(nRBC)可溶性抗原,应用P.v感染者、正常对照混合血清和M26-32单抗与相应的抗原进行免疫印迹分析。结果免疫印迹分析表明,P.v感染者能特异性识别26k、33、49、115kDaP.v抗原;100、102、110、150、175kDaP.f抗原;能够交叉识别的条带有:31、59、63、70、120kDa。M26-32单抗能识别P.f、P.v抗原中31kDa等抗原条带。结论P.v感染者能特异识别间日疟抗原组份,并能交叉识别P.f抗原,其中31kDa抗原组分具有较强的免疫原性,同时能被M26-32单抗识别,其作为P.v特异性诊断抗原的价值有待于进一步研究。

Immunoreactivity comparison between Plasmodium vivax antigen and P.falciparum antigen

To compare and analyze the immunoreactivity difference between Plasmodium vivax antigen and P. falciparum antigen by using immunoblotting with P. v patient＇s sera and pan-species specific Monoclonal antibody M26-32, and to find potentially diagnostic antigen for malaria. Plasmodipur filtration and 60% Percoll density gradient centrifugation techniques were used to purify parasite infected RBC from patient blood. Soluble antigen of P. f and P. v were prepared followed by immunoblotting analysis with P. v patient＇s sera and M26 32 McAb as primary antibody. Immunoblotting results showed that pool P. v patient＇s sera could specifically recognize 26, 33, 49, 115 kDa P. v antigen band, 100, 102, 110,150, 175 kDa P. f antigen band, and cross-recognized 31, 59, 63, 70, 120kDa of both P. v and P. f antigen band. It is concluding that pool P. v patient ＇s sera can specifically recognize P. v antigen and cross-recognize P. f antigen. Furthermore, 31 kDa antigen has strong immunogenity and can be recognized by M26-32 McAb, the potential diagnostic value to human malaria need further study.