茯苓甘草复方合剂对铝染毒小鼠生化指标影响的实验研究

目的研究茯苓甘草复方合剂对铝染毒小鼠生化指标的影响。方法用氯化铝水溶液给小白鼠灌胃3个月,复制染毒模型,然后分为治疗组、染毒组(染毒不治疗组),另设正常组(不染毒,作正常对照)。治疗组用茯苓甘草复方合剂灌胃治疗。实验结束,取血清、大脑组织测定生化指标。结果正常组、治疗组、染毒组乙酰胆碱酯酶(AchE)活力分别是:(0.74±0.18)I U/mg、(0.88±0.13)I U/mg、(1.36±0.18)I U/mg,染毒组明显高于其余2组(P<0.01);实验前、后测Hb结果分别是:(158.42±11.84)g/L、(163.40±7.85)g/L、(161.80±10.02)g/L、(144.16±5.44)g/L、(142.77±23.50)g/L、(128.81±15.90)g/L,染毒组实验结束明显降低(P<0.01);尿素氮(BUN)分别是(7.14±1.51)mmol/L、(8.62±3.53)mmol/L、(10.47±3.29)mmol/L,染毒组明显高于正常组(P<0.05)。结论铝染毒小鼠大脑AchE活力和BUN明显升高,Hb明显降低,而治疗组有明显恢复。

Influence experimental study of Fulinggancao compound mixture on biochemical indicator in aluminum-poisoned mice

Objective It is to study the influence of Fulinggancao compound mixture on the biochemical indicator in aluminum-poisoned mice.Methods Aluminum-poisoned mouse model was established with aluminum chloride water solution intragastric administration for three months,and then the mice were divided into treatment group,poisoned group(poisoned but untreated) and control group(not poisoned,as normal control).The treatment group was treated with Fulinggancao compound mixture.When the experiment finished,the serum and brain tissue were got to determine the biochemical indicator.Results The activity of acetylcholine esterase(AchE) in normal group,treatment group and poisoned group was(0.74±0.18) IU/mg,(0.88±0.13) IU/mg and(1.36±0.18) IU/mg respectively,which in poisoned group was obviously higher than that in the other two groups(P<0.01).Hemoglobin(Hb) in the three groups before and after experiment was(158.42±11.84) g/L,(163.40±7.85) g/L,(161.80±10.02) g/L,(144.16±5.44) g/L,(142.77±23.50) g/L and(128.81±15.90) g/L respectively,which in poisoned group decreased obviously when experiment ended(P<0.01).Blood urea nitrogen(BUN) in the three groups was(7.14±1.51) mmol/L,(8.62±3.53) mmol/L and(10.47±3.29) mmol/L respectively,which in poisoned group was obviously higher than that in control group(P<0.05).Conclusion The AchE activity in brain tissue and BUN increases obviously and Hb decreases obviously in aluminum-poisoned mice, however treatment group has obviously recovered.