化疗药诱导卵巢癌细胞凋亡的初步探讨

目的 观察人体内化疗药诱导卵巢癌细胞凋亡及其规律性。方法 采用末端脱氧核苷酰的转移酶方法,对９例卵巢癌腹水患者行腹腔化疗,观察不同时间腹水中卵巢癌细胞凋亡的动态变化,并应用免疫组化法动态检测腹水中肿瘤细胞增殖及Ｂｃｌ－２,Ｂａｘ基因表达。结果 发现化疗后,肿瘤细胞凋亡多在化疗后４８小时达高峰,与化疗前有显著差异（Ｐ〈０．０５）,至１２０小时,凋亡逐渐下降,部分病例降至治疗前水平,化疗后腹腔内肿瘤细

Primary study of anticancer drug-induced apoptosis in ovarian cancer

Objective: To observe anticancer drug - induced apoptosis and regularizationin ovarian carcinoma. Methods:In our study apoptosis of tumor cells of nine patients with ovarian carcinoma and ascites was evaluated by TDT(terminal deoxynucleotidy transferase)assay. Apoptotic cells in ascites were observed dynamically and regularly after abdominalcavity chemotherapy. We used the techniques of immunohistochemistry to detect the proliferation and protein expression of Bcl - 2 and Bax gene in tumor cells. Results:Apoptotic cells inascites were observed after abdominal cavity chemotherapy and the apoptosis rate peaked "in48 hours after the treatment. This differed greatly from apoptosis before chemotherapy(P<0. 05). Then apoptosis rate gradually declined in 120 hours after the treatment. but did notreach the normal level. The relativion rate (tumor cells/total cells)in ascites decreased afterthe chemotherapy and was inversely correlated with apoptosis. Chemotherapy may not lead toregular proliferation changes. Canclusions: The present paper suggested that chemotherapeutic agents could induce apoptosis in ovarian carcimoma,and further proved that inducingapoptosis may be one of the main mechanisms through which chemotherapeutic agents killcancer cells. TDT can objectively direct apoptosis of ovarian carcinoma, serving as experimental indication for evaluating clinical chemotherapeutic effects.