| Bex2对人脑胶质瘤干细胞增殖、生长和凋亡影响的体外实验研究 |
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| 引用本文： | 于如同,刘明峰,石琼,陈冲,饶海承. Bex2对人脑胶质瘤干细胞增殖、生长和凋亡影响的体外实验研究[J]. 中国微侵袭神经外科杂志, 2008, 13(7) |
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| 作者姓名： | 于如同 刘明峰 石琼 陈冲 饶海承 |
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| 作者单位： | [1]徐州医学院附属医院神经外科; [2]徐州医学院研究生部; [3]江苏徐州 |
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| 摘 要： | 目的观察Bex2真核表达载体对脑胶质瘤干细胞增殖、生长和凋亡的影响,为胶质瘤治疗提供理论基础。方法采用免疫磁珠系统分离、Nestin免疫组化鉴定人脑胶质瘤干细胞;将RT-PCR法获得的Bex2与质粒pcDNA3.1(+)连接,脂质体法转染胶质瘤干细胞,于转染18h、36h、48h、72h、96h后,采用Western Blot检测Bex2表达,MTT法及Hoechst染色试剂盒检测生长抑制率和凋亡情况。结果分离、培养出人脑胶质瘤干细胞,并成功构建Bex2的真核表达载体。转染后18hBex2即有表达,48h达到高峰,后渐下降;转染36h、48h、72h、96h时,细胞生长抑制率和凋亡率明显高于空载体组和空白对照组(P<0.05);转染72h时,细胞生长抑制率和凋亡率最高(P<0.05)。结论Bex2能够抑制脑胶质瘤干细胞的增殖和生长,诱导胶质瘤干细胞凋亡。
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| 关 键 词： | Bex2基因 真核表达载体 胶质瘤干细胞 细胞凋亡 |
Effects of Bex2 gene on multiplication, growth and apoptosis of human brain glioma stem cells in vitro |
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| Affiliation: | YU Rutong1; LIU Mingfeng2; SHI Qiong1; et al. 1. Department of Neurosurgery; Affiliated Hospital of Xuzhou Medical College; Xuzhou 221002; China; 2. Graduated Faculty; Xuzhou Medical College; Xuzhou 221004; |
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| Abstract: | Objective To study effects of Bex2 on multiplication,growth and apoptosis in human glioma stem cells and provide rationale for gene therapy of glioblastoma. Methods Human brain glioma stem cells were isolated and indentified by using the magnetic activated cells sorting (MACS) and nestin immunohistochemistry. The cDNA of Bex2 was amplified by RT-PCR,and then Bex2 ligated to plasmid pcDNA3.1(+). The recombinant was transfected into human glioma stem cells. On 18,36,48,72 and 96 h after transfection,the expre... |
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| Keywords: | Bex2 gene eukaryotic expression vector glioma stem cell apoptosis |
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