畲药食凉茶中三种黄酮类成分含量测定的方法学研究

目的：建立同时测定畲药食凉茶中三种黄酮类成分含量的方法。方法：采用高效液相色谱法测定,色谱柱为Agilent Zorbax SB-C18色谱柱（250 mm×4.6 mm,5μm）;流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速为1.0 ml·min^-1,柱温30℃;检测波长360 nm。结果：芦丁、槲皮素和山柰酚的线性范围分别为0.040 9-1.637 0 mg·ml^-1（r=0.999 2）,0.44-88.00μg·ml-1（r=0.999 8）,0.41-77.63μg·m^l-1（r=0.999 2）;平均加样回收率分别为99.35%（RSD=1.64%）,101.14%（RSD=1.88%）,99.69%（RSD=1.92%）。结论：该方法简便易行、快速准确、重复性好,可用于同时测定食凉茶中的芦丁、槲皮素和山柰酚的含量。

Study on Methodolgy of Content Determination of Three Flavonoids in Traditional She Medicine Shi Liang Cha

Objective： To establish an HPLC method for the simultaneous determination of three flavonoids in Shi Liang Cha. Methods：HPLC was applied with the chromatographic conditions as follows： the chromatographic column was Agilent Zorbax SB-C18 （250 mm × 4. 6 mm, 5μm） at 30℃;acetonitrile-0. 1% H3 PO4 solution was used as the mobile phase with gradient elution; the flow rate was 1. 0 ml·min-1, and the detection wavelength was 360nm. Results： Good linearity of rutin, quercetin and kaempferol was within the range of 0.0409-1.637 0mg·ml-1（r=0.999 2）, 0.44-88.00μg·ml-1（r=0.999 8） and 0.41-77.63μg·ml-1（r=0. 999 2）, respectively; the average recovery was 99.35%（RSD =1. 64%）,101. 14% （RSD =1. 88%） and 99. 69% （RSD =1. 92%） , respectively. Conclusion：The method is simple, accurate and repeatable, and suitable for the simultaneous determination of rutin, quercetin and kaempferol in Shi Liang Cha.