炎消迪娜儿糖浆中五种蒽醌类成分的含量测定

目的：建立炎消迪娜儿糖浆中五种蒽醌类成分含量测定方法。方法：采用HPLC法,色谱柱：DIONEX Acclaim 120A C18（250 mm×4.6 mm,5μm）,以乙腈-0.1%磷酸为流动相进行梯度洗脱,检测波长254 nm,流速：1.0 ml·min^-1;柱温：30℃;进样量：10μl。结果：芦荟大黄素,大黄酸,大黄素,大黄酚和大黄素甲醚分别在0.018 7-0.467 0μg（r=0.999 8）,0.020 2-0.504 0μg（r=0.999 9）,0.013 0-0.390 6μg（r=0.999 9）,0.013 3-0.399 9μg（r=0.999 9）和0.009 3-0.277 8μg（r=0.999 9）范围内线性关系良好,回收率分别为94.30%（RSD=2.68%,n=6）,95.49%（RSD=2.50%,n=6）,93.70%（RSD=2.42%,n=6）,92.52%（RSD=2.40%,n=6）和93.72%（RSD=2.54%,n=6）。结论：该方法准确,灵敏,重复性好,可作为炎消迪娜儿糖浆质量控制的依据。

Determination of Five Anthraquinones Constituents in Yanxiaodinaer Syrups

Objective：To establish the method for the determination of five anthraquinones constituents including aloe-emodin , rhein, emodin , chrysophanol and physcion in Yanxiaodinaer syrups. Methods： An HPLC method was used with a DIONEX Acclaim 120A C18 （250 mm × 4. 6 mm,5 μm） column. The mobile phase was acetonitrile-0. 1% phosphoric acid with gradient elution at flow rate of 1. 0 ml·min-1 . The detection wavelength was 254nm,the column temperature was 30℃and the injection volume was 10 μl. Results：The linear ranges of the five anthraquinones were 0. 018 7-0. 467 0 μg（r=0. 999 8）,0. 020 2-0. 504 0 μg（r=0. 999 9）, 0.013 0-0.390 6 μg（r=0.999 9）,0.013 3-0.399 9 μg（r=0.999 9）and 0.009 3-0.277 8 μg（r=0.999 9）, and the recoveries were 94. 30%（RSD=2. 68%,n=6）,95. 49%（RSD=2. 50%,n=6）,93. 70%（RSD=2. 42%,n=6）,92. 52%（RSD=2. 40%,n=6） and 93. 72%（RSD=2. 54%,n=6）, respectively. Conclusion：The method is accurate , sensitive and reproducible, which can be used as the basis for the quality control of Yanxiaodinaer syrups.