HPLC法同时测定芩翘口服液中黄芩苷、连翘苷的含量

目的：建立同时测定芩翘口服液中黄芩苷、连翘苷含量的方法。方法：色谱柱为Inertsil ODS-SP（250 mm×4.6 mm,5μm）;流动相：磷酸盐缓冲液（取磷酸二氢铵11.5 g,加适量水使溶解,加磷酸1 ml,用水稀释至1 000 ml）-乙腈（75∶25）;流速：1.0 ml·min^-1;柱温：25℃;检测波长：278 nm。结果：黄芩苷线性范围为0.203-4.064μg,r=0.999 8,平均回收率100.9%,RSD为1.7%（n=5）;连翘苷线性范围为0.182-3.648μg,r=0.999 7,平均回收率100.5%,RSD为1.4%（n=5）。结论：该方法简便,准确,重复性好,可用于芩翘口服液中黄芩苷、连翘苷含量的测定。

Content Determination of Baicalin and Phillyrin in Qinqiao Oral Solutions by HPLC

Objective： To establish an HPLC method for the determination of baicalin and phillyrin in Qinqiao oral solutions. Methods：The column was Inertsil ODS-SP（250 mm × 4. 6 mm, 5 μm）. The mobile phase consisted of acetonitrile-phosphate buffer （11. 5 g ammonium dihydrogen phosphate was dissolved in water, and then 1ml phosphoric acid was added, finally the solution was diluted to 1 000 ml by water）（75∶25）. The flow rate was 1. 0 ml·min-1, the detection wavelength was at 278 nm and the column temperature was 25℃. Results：The calibration curve was linear within the range of 0. 203-4. 064 μg（r=0. 999 8） for baicalin and 0. 182-3. 648μg（r=0. 999 7） for phillyrin. The average recovery was 100. 9% with RSD of 1. 7% （n=5） and 100. 5% with RSD of 1. 4% （n=5）, respectively. Conclusion：The method is simple, practicable, accurate and rapid, which can be applied in the determination of baicalin and phillyrin in Qinqiao oral solutions.