| 果糖在微囊化肝细胞制作过程中的细胞保护作用 |
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| 引用本文： | 于聪慧,冷希圣,彭吉润,魏玉华,刘继超,杜如昱. 果糖在微囊化肝细胞制作过程中的细胞保护作用[J]. 中华器官移植杂志, 1999, 20(4): 215-217 |
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| 作者姓名： | 于聪慧 冷希圣 彭吉润 魏玉华 刘继超 杜如昱 |
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| 作者单位： | 1. 北京军区总医院肝胆外科
2. 北京医科大学人民医院外科,100044 |
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| 基金项目： | 国家自然科学基金国际合作项目资助 |
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| 摘 要： | 目的 为了减少肝细胞在微囊化过程中的缺氧损伤，研究在缺氧状态下果糖对肝细胞的保护作用。方法 将ＳＤ大鼠分为三组；Ａ组，细胞分离的灌流液和消化液中均加果糖２０ｍｍｏｌ／Ｌ，Ｂ，Ｃ两组不加果糖；细胞分离及微囊化后，检测细胞的活率，微囊化肝细胞体外培养，Ｃ组培养液中加果糖１０ｍｍｏｌ／Ｌ，Ａ，Ｂ两组不加。
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| 关 键 词： | 缺氧保护 果糖 肝细胞 微囊化肝细胞 细胞保护 |
The protective effect of fructose in the process of microencapsulation of hepatocytes |
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| YU Conghui,LENG Xisheng,PENG Jirun,et al.. The protective effect of fructose in the process of microencapsulation of hepatocytes[J]. Chinese Journal of Organ Transplantation, 1999, 20(4): 215-217 |
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| Authors: | YU Conghui LENG Xisheng PENG Jirun et al. |
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| Affiliation: | YU Conghui,LENG Xisheng,PENG Jirun,et al. People's Hospital,Beijing Medical University,Beijing 100044 |
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| Abstract: | Objective To study the protective effect of fructose with regard to hypoxia induced hepatocytes injury during the long microencapsulation periods. Methods Eighteen SD rats were divided into three groups, 6 rats each. In group A, fructose was added into the perfusion solutions (20?mmol/L). There was no fructose in group B and C. After isolation and microencapsulation, the viability was tested. In vitro culture, fructose (10?mmol/L) was added in culture medium in group C, but not in groups A and B. LDH leakage and TP of culture were examined.Results The survival of hepatocytes in group A was obviously higher than in groups B and C ( P < 0.01 ). The LDH leakage in group A was significantly lower than in groups B and C ( P < 0.01 ).Conclusion Fructose could protect hepatocytes against hypoxia induced cell damage during microencapsulation, but cells should be pre treated with fructose before hypoxia. |
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| Keywords: | Hypoxia protection Fructose Hepatocyte Microencapsulation |
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