| 虫草花与冬虫夏草腺苷成份的比较研究 |
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| 引用本文： | 曾秀兰,黄红中.虫草花与冬虫夏草腺苷成份的比较研究[J].国际医药卫生导报,2009,15(14). |
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| 作者姓名： | 曾秀兰 黄红中 |
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| 作者单位： | 1. 开平市中医院,529300
2. 中山大学附属第一医院,510080 |
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| 基金项目： | 江门市卫生系统科研项目 |
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| 摘 要： | 目的 比较研究虫草花与冬虫夏草腺苷成份的含量.方法 采用高效液相色谱法测定腺苷的含量,色谱柱为岛津Shim-pack VP-ODS柱(200mm×4.6 mm,5μm);流动相为磷酸盐缓冲剂(pH=6.5)-甲醇(17:3);流速为1ml/min;检测波长为260nm.结果 腺苷的线性范围为0.078 35-0.399 88μg,呈现较好的线性关系,平均加样回收率为96.22%,RSD为0.21%(n=5).结论 虫草花的活性成份腺苷含量明显高于冬虫夏草腺苷含量,具有与冬虫夏草相似的滋补功效.
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| 关 键 词： | 冬虫夏草 虫草花 腺苷 |
Comparative study on determination of adenosine in Cordyceps sinensis and Cordyceps Flower |
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| ZENG Xiu-lan,HUANG Hong-zhong.Comparative study on determination of adenosine in Cordyceps sinensis and Cordyceps Flower[J].International Medicine & Health Guidance News,2009,15(14). |
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| Authors: | ZENG Xiu-lan HUANG Hong-zhong |
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| Abstract: | Objective To compare the content difference of adenosine in Cordyceps sineasis and Cordyceps Flower.Methods HPLC method was applied to determine the contents of adenosine,with a column of Shim-pack VP-ODS 4.6 nun×200mm,5μm) and a mobile phase of phosphate buffer solution(pH=6.5)-methanol(17:3),flow rate was 1ml/min,detection wavelength was 260nm.Results The linear range of adenosine was 0.07835-0.39988μg,the result showed good linearity.The average recovery was 96.22%,RSD 0.21%.Conclusion The content of adenosine in Cordyceps Flower was higher.It is suggested that Cordyceps Flower has the same tonic efficacy as Cordyceps sinensis. |
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| Keywords: | HPLC Cordyceps sinensis Cordyceps Flower Adenosine HPLC |
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