建立海人酸颞叶癫模型的实验研究

目的建立用立体定向技术制作大鼠海人酸颞叶癫癎模型的方法,并对模型永久癫癎敏感性的原因进行研究。方法将海人酸通过立体定向手术注入大鼠的海马组织,于不同的时间观察大鼠的癫癎发作情况和海马组织的病理学改变。结果大鼠在经历“湿狗样抖动”、口唇和头的自运动症、前肢抽搐、后肢抽搐后,进入强直-阵挛性全身发作,之后,每周均有自行发作,表现与人类颞叶癫癎极为一致。海马神经元的缺失、胶质细胞增生是模型长期癫癎敏感性的基础。结论立体定向方法建立的大鼠颞叶癫癎模型的发作形式及病理学改变与人类的颞叶癫癎极为一致,并且具有长期的癫癎敏感性。另外,立体定向局部注药所用海人酸的量较系统给药明显减少,花费显著减少。因此,该模型即可靠又经济。

Experimental study of establishing the temporal epilepsy model with kainic acid

Objective To establish the temporal epilepsy models in rat with kainir acid (KA) by stereotactic technique and to explore the pathological basis why the model' s epilepsy existed permanently. Methods KA was injected into the rat's hippocampus by stereotactic operation and the seizures and morphological changes of hippocampus were observed during the different period of time. Rusults After operation, the rats underwent twitter like wet-dog, lip's and head's self-movements, forelimbs' seizures and hind limbs' seizures, then the stupor-clonus seizures.The seizure took place after every week. The manifestations of seizure were almost as same as those of human temporal epilepsy. The loss of hippocampus neurons and the hyperplasia of gliocytes were the basis for the permanent existance of model' s epilepsy . Conclusion The seizure pattern and pathological basis of temporal epilepsy models in rat established by stereotactic management were almost as the same of human beings' . And the models' epilepsy existed permanently. Moreover, the dose of KA of local injection was less than that of systemic injection,and the cost was decreased greatly. As a result, the model was credible and economical.