中药材骨碎补及其混淆品的显微鉴定方法研究

目的:为中药材骨碎补正品及其混淆品提供科学可行的显微鉴定方法。方法:通过光学显微镜、扫描电子显微镜及石蜡切片方法对骨碎补正品的来源植物槲蕨Drynaria roosii及其混淆品来源植物川滇槲蕨D.delavayi、栎叶槲蕨D.quer-cifolia和崖姜Pseudodrynaria coronans的根状茎鳞片的形态特征进行观察、测定并进行数据分析。结果:槲蕨、川滇槲蕨和崖姜之间在鳞片长度方面两两比较均出现显著性差异;川滇槲蕨鳞片锯齿呈不均匀分布且多发生弯曲,有重锯齿现象;槲蕨、栎叶槲蕨和崖姜的鳞片基均着生于表皮凹陷处,而川滇槲蕨鳞片基着生于表皮凸起处。结论:几种材料的根状茎鳞片有显著差异,可以作为鉴别骨碎补正品槲蕨及其混淆品的依据。

Microscopic authentication method of traditional Chinese medicine Gusuibu

Objective : To provide practical method for microscopic authentication of traditional Chinese medicine Gusuibu and its adulterants. Method : By means of light microscope, scanning electron microscopy and tissue section techniques, the morphology, the size of the rhizome scales and their bearing position in the original plants of Gusuibu and its adulterants, i.e. Drynaria roosii, D. delavayi, D. quercifolia and Pseudodrynaria coronans were analyzed. Result : There were significant differences between scales length of D. roosii, D. delavayi and P. coronans, while there was no significant difference between that of D. roosii and D. quercifolia. The scale teeth of D. delavayi were usually curved, bifid and uneven distributed at the scale fringe, which was different from that of the other three species. The base of the scales sinks in epidermis in D. roosii, D. quercifolia, and P. coronans, while it bore at the raised part of epidermis in D. delavayi. Conclution : Morphology, size and bearing position of the rhizome scales have significant differences in the several species. Therefore, these characteristics can be applied to the identification of Gusuibu and its adulterants.