| 化学计量学方法研究北五味子中木脂素含量与抗氧化活性的相关性 |
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| 引用本文： | 皮子凤,侯广月,艾军,宋凤瑞,刘志强,刘淑莹. 化学计量学方法研究北五味子中木脂素含量与抗氧化活性的相关性[J]. 中国中药杂志, 2012, 37(8): 1133-1139 |
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| 作者姓名： | 皮子凤 侯广月 艾军 宋凤瑞 刘志强 刘淑莹 |
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| 作者单位： | 1. 中国科学院长春应用化学研究所长春质谱中心,吉林长春,130022
2. 中国农业科学院特产研究所,吉林长春,132109 |
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| 基金项目： | 国家自然科学基金项目(81073040);吉林省重点科技发展项目(20090908) |
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| 摘 要： | 目的:通过五味子抗氧化活性与木脂素含量的相关性分析,建立五味子抗氧化活性的快速评价方法。方法:采用高效液相色谱测定了采自吉林省不同地区的37批不同五味子样品中的5种木脂素的含量,并分别从抑制肝、肾、脑中脂质过氧化能力和清除.DPPH自由基能力几方面测定了上述样品的抗氧化活性。以SPSS 11.5软件进行了双变量相关性(biva-riate)分析及多元线性回归的逐步回归(stepwise)分析。结果:双变量相关性分析结果表明,五味子醇甲与抑制肝脏、脑脂质过氧化、.DPPH清除能力具有显著的相关性(P<0.01),均呈正相关;五味子醇乙与4种抗氧化活性均有相关性(P<0.05或0.01),均呈正相关;而五味子甲素与抑制肝脏、脑脂质过氧化、.DPPH清除能力具有显著的负相关性(P<0.01),五味子乙素仅与抑制脑脂质过氧化能力具有负相关性(P<0.05)。逐步回归分析结果与Bivariate分析结果基本一致,并分别得出木脂素与不同模型中抗氧化活性相关性的线性回归方程。采用另外10批样品的测定结果经回归方程模拟,所得的抗氧化活性与测定的抗氧化活性基本一致。结论:采用相关性分析和线性逐步回归分析法建立的抗氧化活性评价模型,从而确定五味子抗氧化活性的物质基础,同时还可根据所测定的五味子中木脂素的含量来推测样品的抗氧化活性。
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| 关 键 词： | 五味子 木脂素 抗氧化活性 化学计量学 相关性分析 |
| 收稿时间： | 2011-11-07 |
Correlation of lignans content and antioxidant activities of Schisandra chinensis fruits by using stoichiometry method |
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| PI Zifeng,HOU Guangyue,AI Jun,SONG Fengrui,LIU Zhiqiang and LIU Shuying. Correlation of lignans content and antioxidant activities of Schisandra chinensis fruits by using stoichiometry method[J]. China Journal of Chinese Materia Medica, 2012, 37(8): 1133-1139 |
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| Authors: | PI Zifeng HOU Guangyue AI Jun SONG Fengrui LIU Zhiqiang LIU Shuying |
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| Affiliation: | Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, China;Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, China;Institute of Special Wild Economic Animal and Plant, The Chinese Academy of Agricultural Sciences, Changchun 132109, China;Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, China;Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, China;Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, China |
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| Abstract: | Objective : To establish a quick method for evaluation of the antioxidant activities based on the correlation analysis of lignans content and antioxidant activities of Schisandra chinensis fruits. Method : The content of five lignans components in 37 batches of S. chinensis fruits from different regions of Jilin province were measured by HPLC. Simultaneously, the antioxidant activities of the above samples were detected, such as lipid peroxidation inhibition activity in liver(LPIL), kidney(LPIK) and brain(LPIB)and the clearance rate of DPPH (CRD). Bivariate correlation analysis and stepwise regression analysis were carried out by the software of SPSS for windows 11.5. Result : The results of bivariate correlation analysis showed that deoxyschizandrin was negative correlation (P<0.01) to the activity of LPIL, LPIB, CRD. Schisandrin was positive correlation (P<0.01) to the activity of LPIL, LPIB, CRD. Schisandrol B was also positive correlation (P<0.05 or P<0.01) to the above four kinds of antioxidant activity. The results of stepwise regression analysis were mostly consistent with the bivariate correlation analysis results. For the other 10 batches of samples, the simulated antioxidant activities according to the regression equation calculated was consistent with the measured activities. Conclusion : By using the bivariate correlation analysis and linear stepwise regression analysis, the bioactive components related to the antioxidant activity of S. chinensis fruits were found. Meanwhile, the antioxidant activity of samples will be inferred according to the content of Schisandra lignans. |
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| Keywords: | Schisandra chinensis fruits lignans antioxidant activity stoichiometry correlation |
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