| As_2O_3逆转K_(562/ADM)细胞多药耐药的研究 |
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| 引用本文： | 王淑婷,李清,刘超,赵谨瑶,于丽敏,卢步峰,杨佩满. As_2O_3逆转K_(562/ADM)细胞多药耐药的研究[J]. 解剖科学进展, 2002, 8(3): 237-239 |
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| 作者姓名： | 王淑婷 李清 刘超 赵谨瑶 于丽敏 卢步峰 杨佩满 |
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| 作者单位： | 1. 大连大学医学院,辽宁,大连,116622
2. 大连医科大学省细胞分子生物学重点实验室,辽宁,大连,116027 |
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| 摘 要： | 目的 阐述As2 O3 (三氧化二砷 )治疗人红白血病机制。方法 采用人红白血病多药耐药株K562 / ADM作为实验模型 ,研究As2 O3 对该细胞MDR逆转的可能性。结果 ①K562 / ADM 细胞对ADM具有明显的抗性 ,与K562 细胞相比 ,抗性倍数约为 4 0倍 ,而两者对As2 O3 的IC50 接近 ,无显著差异 ;②低毒剂量的As2 O3 (1 0 μmol/L)与ADM(4 0 μg/ml)联合运用 ,可升高细胞内ADM的浓度 ,降低细胞对ADM的IC50 ,使该细胞对ADM的抗性有数倍逆转。结论 As2 O3 是一种有效的MDR逆转剂。
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| 关 键 词： | 三氧化二砷 多药耐药性 人红白血病细胞 |
| 文章编号： | 1006-2947(2002)03-0237-03 |
| 修稿时间： | 2002-04-09 |
Reversal Effect of As2 O3 on Multidrug Resistance K562/ADM Cell Line |
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| WANG Shu-ting,LI Qing,LIU Chao,et al. Reversal Effect of As2 O3 on Multidrug Resistance K562/ADM Cell Line[J]. Progress of Anatomical Sciences, 2002, 8(3): 237-239 |
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| Authors: | WANG Shu-ting LI Qing LIU Chao et al |
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| Abstract: | Objective To illustrate mechanisms of arsenic trioxide (As 2O 3) in the treatment of K 562 / ADM cells. Methods We study the reversal effect of As 2O 3 on K 562 / ADM MDR cells in experiment model of rat. Results ① The resistance of K 562 / ADM cell line to ADM was 40 times than that of K 562 cell line, but the IC 50 value of both cell lines to As 2O 3 was similar (P>0.05); ② low cytotoxic dose (2.0 μmol/L) of As 2O 3 in combination with ADM (4.0 μg/ml) increased the intracellular ADM concentration (P<0.05) and declined IC 50 value of K 562 / ADM cell line to ADM by 2.5 times than the control. Conclusion As 2O 3 might be an effective anti tumor drug as well as an effective drug for reversal of MDR. |
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| Keywords: | As 2O 3 multidrug resisatnce human erythroleukemia cells |
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