去颗粒方法对卵母细胞冻融的影响

目的:探讨去除卵母细胞周围部分颗粒细胞对冻融后卵母细胞发育潜能的影响。方法:将来源于行体外受精-胚胎移植(IVF-ET)患者捐赠的43枚多余成熟卵母细胞(MⅡ期)随机分为A、B两组,在不同条件下进行慢速冷冻法冻融。根据不同的去除卵母细胞周围部分颗粒细胞的方法将卵母细胞分为两组,A组:21枚,透明质酸酶消化法去颗粒;B组:22枚,机械法去颗粒。观察不同的去颗粒方法对冻融后的卵母细胞存活率、受精能力及胚胎早期发育力的影响。结果:A组存活率(52.38%vs 77.27%)、受精率(63.64%vs 70.59%)及卵裂率(57.14%vs 75.00%)均略低于B组,但无显著性差异;两组优质胚胎率(50.00%vs 55.56%)、桑葚胚率(25.00%vs 22.22%)及囊胚率(25.00%vs 11.11%)也均无显著性差异(P>0.05)。结论:不同的去颗粒方法不影响卵母细胞冻融后的发育潜能。

Effect on the development potential of human frozen -thawed oocyte by different degranulation methods

Objective:To explore the effect on the development potential of human frozen-thawed oocyte by different degranulation methods.Methods:A total of 43 mature oocytes were obtained from consented infertility patients undergoing IVF-ET treatment,who donated their superfluous oocytes.These oocytes were divided into two groups randomly and frozen-thawed in different conditions to compare these groups' developmental potential in different degranulation methods.Results:No significant differences were found in the rate of post-thaw survival(52.38% vs 77.27%),fertilization(63.64% vs 70.59%),cleavage(57.14% vs 75.00%),high-quality embryo(50.00% vs 55.56%),morula(25.00% vs 22.22%) and blastocyst(25.00% vs 11.11%) between two group A and group B(P>0.05).Conclusion:The development potential of post-thaw oocyte is not effected by different degranulation methods.