肝移植对肝硬化大鼠脾功能及脾组织形态学的影响

目的探讨肝移植对肝硬化大鼠脾功能及脾组织形态学的影响。方法制备四氯化碳中毒性肝硬化大鼠模型，采用“二袖套法”进行肝移植。观测肝移植前后大鼠门静脉压力（portal vein pressure，PVP）以及脾脏组织形态学的变化；检测肝移植前后大鼠血清中促吞噬肽（tuftsin）含量的变化。结果肝移植前，肝硬化大鼠PVP从6．28mmHg（1mmHg=0．133kPa）升至14．03mmHg（P＜0．01）；脾脏病理切片示白髓面积从23．47％缩小至7．70％（P＜0．01）；脾小梁面积从1．75％扩大至4．46％（P＜0．01）。血清tuftsin含量自466．7ng／mL减少至321．8ng／mL（P＜0．01）。肝移植后，随着时间的延长，PVP从14．03mmHg明显降至6．33mmHg（P＜0．01）。脾脏病理切片示白髓面积从7．70％扩大至15．07％（P＜0．01）；脾小梁面积从4．46％缩小至3．11％（P＜0．05）。血清tuftsin含量从321．8ng／mL升至432．5ng／mL（P＜0．01）。结论肝硬化大鼠肝移植后门静脉高压症可明显缓解，异常的脾功能逐渐得到改善。

Effects of liver transplantation on function and morphological changes of spleen in hepatic cirrhosis rat

Objective To investigate the effects of liver transplantation on function and morphological changes of spleen in hepatic cirrhosis rats.Methods Hepatic cirrhosis model was established in rats by subcutaneous injections of carbon tetrachloride.Liver transplantation model was established with two-cuff technique.The portal vein pressure,and morphological changes of spleen were observed before and after liver transplantation in hepatic cirrhosis rats.The serum tuftsin level before and after liver transplantation were detected.Results Before liver transplantation,PVP was increased from 6.28 mmHg to 14.03mmHg(P<0.01).(Pathological) sections of spleen showed that the areas of white pulp were decreased from 23.47% to 7.70%(P<(0.01)),and the areas of spleen trabecula were increased from 1.75% to 4.46%(P<(0.01)).Meanwhile,the level of serum tuftsin was significantly decreased from 466.7ng/mL to 321.8ng/mL(P<0.01).After liver transplantation,PVP was significantly decreased from 14.03mmHg to 6.33mmHg(P<0.01).Pathological sections of spleen showed that the areas of white pulp were increased from 7.70% to 15.07%(P<0.01),and the areas of spleen trabecula were decreased from 4.46% to 3.11%(P<0.05).Meanwhile,the level of serum tuftsin was increased from 321.8ng/mL to 432.5ng/mL(P<(0.01)).Conclusions Portal vein hypertension in hepatic cirrhosis rats could be relieved after liver transplantation.Liver transplantation can improve the impaired function of spleen.