复方心康口服液心肌保护作用的实验研究

目的:探讨心康口服液对急性心肌损伤的保护作用。方法:(1)将小鼠分为对照组、运动衰竭组(运衰组)、牛磺酸 运衰组(牛磺酸组)、丹参 运衰组(丹参组),采用不同比例配伍的心康口服液用于小鼠,观察其游泳至衰竭时间。(2)用Wistar大鼠建立离体心脏模型及心脏损伤模型,分为对照组、缺血损伤(AR)组、心康组,分别观察各组大鼠心功能指标、CPK活性、心肌组织MDA及GSHPX,SOD活性、心肌细胞超微结构。(3)以1～3d的SD大鼠的心室肌细胞为基质,建立心肌细胞缺氧复氧损伤模型,分为对照组(复氧组)、AR(缺氧复氧)组、心康组,观察3组的心肌细胞存活率、培养液中LDH活性、心肌细胞超微结构。结果:(1)牛磺酸组、丹参组较衰竭组小鼠的游泳时间显著延长(P<0.05),血清中CPK活力低于运衰组(P<0.05)。心康口服液中牛磺酸对小鼠的游泳能力影响较大,且高浓度好;丹参的影响次之,中浓度较好。(2)对照组整个灌流期间LVSP、dpdtmax、HR均无明显变化,心肌细胞超微结构正常。AR组在复灌5、10、20、30min时LVSP、dpdtmax、HR下降(P<0.01),心肌酶活力降低,MDA含量及CPK活性升高(P<0.01),心肌细胞超微结构破坏,成不可逆损伤,心康组在复灌5、10、20、30min时LVSP、dpdtmax、HR与AR组相比均升高(P<0.01);心肌酶活力均升高,MDA含量及CPK活性降低(P<0.01);细胞超微结构大有改善。(3)AR组与对照组相比其细胞存活率降低(P<0.01),LDH活力升高(P<0.01),心康组与AR组相比细胞存活率升高(P<0.01);LDH活力降低(P<0.01);对照组心肌细胞超微结构正常,AR组心肌细胞超微结构破坏,成不可逆损伤,心康组细胞超微结构大有改善。结论:牛磺酸和丹参配物而成的心康口服液,对心肌缺血再灌注损伤具有显著的保护作用,心康口服液作为心肌缺血再灌注损伤治疗的辅助性用药将具有较好的临床应用前景。

Experimental study of protective effects of compound oral liquid xinkang on myocardium

AIM: To explore the protective effects of compound oral liquid xinkang on the acute myocardial injury. METHODS: The rats were divided into four groups: control group, sport group, Tau group and Ts group. 9 groups of rats were taking compound oral liquid xinkang of different ingredient, and the swimming time was observed. Exosomatic heart model of Wistar rats were divided into three groups: control group, A/R group and xinkang group. Heart function index, CPK activity, MDA, GSH-PX, SOD and myocardial microscopic structure were observed. The ventricular myocardial cells of 1-3 days old SD rats were divided into three groups. In control group, the cells were cultivated with reperfusion liquid. In A/R group, the cells were cultivated with anoxia/reperfusion liquid. In xinkang group, the cells were first cultivated with xinkang oral liquid and second with anoxia/reperfusion liquid. Myocardial survival ratio, LDH activity in cultivating liquid and myocardial microscopic structure were observed. RESULTS: Swimming time in Tau and Ts group were longer than that in sport group (P<(0.05)). Serum CPK in Tau and Ts group were lower than that in sport group (P<(0.05)). Taurine influenced the swimming time much more than salvia miltiorrhiza. Taurine of high concentration and salvia miltiorrhiza of middle concentration were better. LVSP, dp/dt_(max) and HR in control group were steady during perfusion, but those in A/R group were decreased during perfusion for 5, 10, 20 and 30 min (P<(0.01)), and those in xinkang group were higher than those in control group during perfusion for 5, 10, 20 and 30 min (P<(0.01)). In A/R group, myocardial enzyme activity was lower, and MDA and CPK were higher than those in control group (P<(0.01)). The results were inverse contrast xinkang group with A/R group. Myocardial microscopic structure in control group was normal, and that in A/R group was seriously destroyed. The structure in xinkang group was improved. Myocardial survival ratio in A/R group was lower than that in control group (P<(0.01)). The ratio in xinkang group was higher than that in A/R group (P<(0.01)). LDH activity in A/R group was higher than that in control group (P<0.01).The activity in xinkang group was lower than that in A/R group (P<(0.01)). Myocardial microscopic structure in control group was normal, and that in A/R group was seriously destroyed. The structure in xinkang group was improved. CONCLUSION: Oral liquid xinkang compounded of taurine and salvia miltiorrhiza can protect the myocardium experienced anoxia/reperfusion.