不同剂量海人藻酸致痫鼠海马各亚区病理变化的比较

目的：通过观察海人藻酸（KA）致痫鼠在KA不同剂量条件下其海马各亚区病理变化特点，探讨癫痫的信号传导通路及发病机制。 方法：Wistar雄性大鼠30只，随机分为正常对照组、小剂量KA组（0.025 μg）和大剂量KA组（0.100 μg）（每组10只），用微管注射法在右侧杏仁核内注射KA制备癫痫模型，观察不同剂量KA引起的海马各亚区病理变化特点。 结果：与正常对照组比较， 大剂量KA组大鼠海马区表现为以CA3区细胞脱落为主，CA1区锥体细胞及齿状回颗粒细胞大小形态正常，排列整齐。小剂量KA注射则使CA1和CA3区细胞均受损，而齿状回细胞始终保持完好。 结论：KA致痫鼠海马各亚区病理变化随KA剂量不同而不同，可能与癫痫信号传导的顺序及海马自身的特性有关。

Comparison of pathological changes of hippocampal pyramidal subregions in rats with epilepsy induced with different doses kainitic acid

Objective To investigate the charateristics of pathological changes in hippocampal subregions in rats with epilepsy induced with different doses kainic acid(KA) ,discuss the etiology and pathway of epileptic wave.Methods 30 male Wistar rats were randomly divided into control group,low(0.025 μg) and high(0.1 μg) dose KA injection groups with 10 rats each.KA was focally injected into the right amygdala by a glass micropipette connected to an air pressure system to make epilepsy model.The pathological characteristics in hippocampal subregions in rats with epilepsy induced with different doses KA were observed.Results Compared with control group,high dose KA injection mainly caused neuron loss in the CA3 region,while pyramidal and dentate granule cells were evenly distributed with normal shape and size.Low dose KA injection caused severe damage in both CA1 and CA3 regions.Dentate granule cells didn't show any pathological change and neuron loss in low dose injection.Conclusion The pathological changes in hippocampal subregions in rats with epilepsy induced with KA are different with different doses KA,it might be related to the pathway of epileptic wave and the specific properties of hippocampus.