大肠腺癌中iNOS和COX-2的表达及意义

目的 探讨大肠腺癌中iNOS和COX-2的表达及其与大肠腺癌生物学行为的关系.方法 采用SP免疫组化方法检测78例大肠腺癌标本及33例癌旁正常肠黏膜组织中的iNOS和COX-2的表达,并对两指标表达与临床病理参数之间进行相关分析.结果 ① iNOS和COX-2在大肠腺癌中阳性率分别显著高于正常大肠黏膜阳性率(P<0.05);② iNOS、COX-2的表达与大肠癌淋巴结转移明显相关(P<0.05);③ iNOS和COX-2在大肠腺癌中的阳性表达与临床分期有关,在TNM分期中Ⅲ+Ⅳ期阳性表达率高于Ⅰ+Ⅱ期(P<0.05);④ 大肠腺癌组织中COX-2表达与iNOS表达之间存在明显相关性(P<0.05).结论 COX-2和iNOS过度表达参与了大肠腺癌的发生发展过程,并与其淋巴结转移和TNM分期有关;大肠腺癌中COX-2和iNOS的表达具有正向协同性.

Expression of iNOS and COX-2 and their significance in colorectal adenocarcinoma

Purpose To study the expression and interaction of iNOS and COX-2 in colorectal adenocarcinoma, as well as their relationship with the biological behaviors of colorectal adenocarcinoma.Methods Intestinal biopsy specimens of colorectal adenocarcinoma were collected in the 78 cases and 33 normal intestinal mucosal tissues.The expression of iNOS and COX-2 proteins was detected by immunohistochemical staining (SP method).Results The positive rates of iNOS and COX-2 protein was significantly higher in normal intestinal mucosa than that in colorectal adenocarcinoma (P<0.05).The expression of iNOS and COX-2 protien had significant relation with lymph node metastasis (P<0.05).The positive expression of iNOS and COX-2 in intestinal adenocarcinoma was related with TNM stage:the positive expression in patients with Ⅲ+Ⅳ stage was higher than that with Ⅰ+Ⅱstage (P<0.05). The expression of iNOS was closely correlated with COX-2 (P<0.05).Conclusions The overexpression of COX-2 and iNOS participates in the pathogenesis of colorectal carcinoma and is associated with lymph node metastasis and TNM stage of colorectal adenocarcinoma.The expression of iNOS is correlated with COX-2 in the cancer.