| 三联放大技术与荧光定量PCR检测乙型肝炎病毒的应用比较 |
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| 引用本文： | 唐九歌,丁剑冰,郭劲,乔艳辉,袁晓华,何智,郭伟鹏. 三联放大技术与荧光定量PCR检测乙型肝炎病毒的应用比较[J]. 中国病原生物学杂志, 2010, 0(1) |
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| 作者姓名： | 唐九歌 丁剑冰 郭劲 乔艳辉 袁晓华 何智 郭伟鹏 |
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| 作者单位： | 新疆医科大学基础医学院;乌鲁木齐血液中心; |
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| 基金项目： | 新疆高技术研发项目(No.20061110,PT0805) |
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| 摘 要： | 目的通过比较聚合酶链反应偶联印迹杂交和酶联放大技术(即三联放大技术)与荧光定量PCR(FQ-PCR)两种方法检测HBV DNA结果,探讨三联放大技术用于血液HBV筛查的优越性。方法医院肝病门诊患者血清195份,同时进行三联放大技术检测和FQ-PCR检测,比较检测结果。结果195份血清,经三联放大技术检出HBVDNA 125份,检出率64.10%;FQ-PCR检出HBV DNA 103份,检出率52.82%。两种方法的HBV DNA检出率差异有统计学意义(P0.01)。结论与FQ-PCR比较,三联放大技术能显著提高HBV DNA的检出率,可用于血液HBV筛查。
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| 关 键 词： | 三联放大技术 荧光定量PCR 肝炎病毒 乙型 血液 检测 |
Comparing PCR coupled with blot-hybridization and enzyme linking to FQ-PCR to detect HBV |
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| TANG Jiu-ge,DING Jian-bing,GOU Jing,QIAO Yan-hui,YUAN Xiao-hua,HE Zhi,GUO Wei-peng. Comparing PCR coupled with blot-hybridization and enzyme linking to FQ-PCR to detect HBV[J]. Journal of Pathogen Biology, 2010, 0(1) |
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| Authors: | TANG Jiu-ge DING Jian-bing GOU Jing QIAO Yan-hui YUAN Xiao-hua HE Zhi GUO Wei-peng |
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| Affiliation: | TANG Jiu-ge1,DING Jian-bing1,GOU Jing2,QIAO Yan-hui2,YUAN Xiao-hua2,HE Zhi2,GUO Wei-peng2 (1.College of Basic Medicine,Xinjiang Medical University,Urumqi 830054,China,2.Blood Center,Xinjiang,Urumqi 830000,China) |
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| Abstract: | Objective To compare the results of HBV DNA detection with two techniques: polymerase chain reaction coupled with blot-hybridization and enzyme linking(the Triple Amplification Technique) and fluorescence quantitative PCR(FQ-PCR).Discussion The Triple Amplification Technique detected HBV in screened blood donors.Methods The Triple Amplification Technique and FQ-PCR detected 195 serum samples from hepatitis patient in clinic of the hospital,then compared to testing result.Results The Triple Amplification Tec... |
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| Keywords: | Triple Amplification Technique FQ-PCR HBV blood detection |
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