Modulation of morphology and functions of human hepatoblastoma cells by nano-grooved substrata |
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| Authors: | Wei-Bor Tsai Jia-Hua Lin |
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| Affiliation: | 1. Research Lab for Simulation and Process Control, Department of Chemical Engineering, Iran University of Science and Technology, Narmak, Tehran 16846-13114, Iran;2. Young Researchers and Elite Club, South Tehran Branch, Islamic Azad University, Tehran, Iran;1. Department of Chemical and Materials Engineering, Tunghai University, Taichung 407, Taiwan;2. Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan;3. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan;4. University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan;1. University Montpellier, CNRS UMR 5235, Dynamique des Interactions Membranaires Normales et Pathologiques, 34095 Montpellier, France;2. CNRS UMR 5048, University Montpellier, Centre de Biochimie Structurale, 34090 Montpellier, France;1. Twin Cities Orthopedics, 4010 West 65th Street, Edina, MN 55435, USA;2. The Foot and Ankle Association Inc., Institute for Foot and Ankle Recon at Mercy, 301 Saint Paul Place, Baltimore, MD 21202, USA;3. Orthopaedic Associates of Michigan, 230 Michigan Northeast, Suite 300, Grand Rapids, MI 4950, USA;1. Department of Chemical Engineering, National Taiwan University of Science and Technology, 43, Keelung Road, Sec. 4, Taipei 106, Taiwan;2. The Food Industry Research and Development Institute (FIRDI), 331, Shih-Pin Road, Hsinchu 300, Taiwan;3. Department of Chemical Engineering, National Taiwan University, Sec. 4, No. 1, Roosevelt Road, Taipei 106, Taiwan |
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| Abstract: | It is known that cellular behavior is affected by nano-patterned topography. For example, many cell types tend to align and extend along the direction of nano-grooves/ridges structures. In this study, we investigated the impact of nano-grooves/ridges on hepatocyte morphology and functions. HepG2/C3A (C3A) cells were cultured on nano-grooved silicon or polystyrene substrata with various widths (from 100 to 500 nm) and depths (from 100 to 380 nm). Nano-grooved substrates induced dramatic changes in C3A cell morphology. The cells formed spheroids on the flat substrates, while C3A cells spread and grew confluently with elongated and aligned morphology along the nano-grooves/ridges. Albumin synthesis was enhanced on the nano-grooved silicon substrates compared to the flat surface, and was decreased with increasing groove depths. Urea conversion on the shallow grooves (400 nm wide and 100 nm deep) remained at the same level of that on the flat surfaces, but was decreased on the deeper grooves. We found that the functions of hepatocytes were enhanced on the substrates with shallow grooves. The nano-grooved substrates may be applied as in vitro culture systems of hepatocytes for both diagnostic and therapeutic applications. |
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