| 通痹灵总碱对活化的小鼠T淋巴细胞CD69表达的影响 |
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| 引用本文： | 陈纪藩,陈光星,李晓娟,全世明,曾耀英,胡英杰,黄可儿,刘晓玲.通痹灵总碱对活化的小鼠T淋巴细胞CD69表达的影响[J].细胞与分子免疫学杂志,2003,19(1):93-94. |
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| 作者姓名： | 陈纪藩 陈光星 李晓娟 全世明 曾耀英 胡英杰 黄可儿 刘晓玲 |
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| 作者单位： | 1. 广州中医药大学第一附属医院六内科,广东,广州,510405
2. 暨南大学生命科学院组织移植与免疫中心,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金重点项目资助(No. 39930230)，广东省中医药管理局课题资助(No.102019) |
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| 摘 要： | 目的：研究通痹灵（TBL）总碱对活化的小鼠T淋巴细胞表达CD69的影响及其可能的免疫调控机制。方法：培养小鼠淋巴细胞，加入不同浓度的TBL总碱预孵1h后，再加佛波醇酯（PDB）或刀豆蛋白（ConA).24h后，用流式细胞术检测T淋巴细胞CD69的表达率。结果；不同质量浓度的TBL总碱对PDB或ConA激活的T淋巴细胞表达CD69，均有明显的下调作用（对ConA激活的T淋巴细胞表达CD69。均有明显的下调作用（对ConA激活的T淋巴细胞的作用要强于PDB激活的T淋巴细胞）。呈明显的量效关系。结论：TBL总碱可明显抑制活化的小鼠T淋巴细胞CD69的表达，为其用于类风湿性关节炎的治疗提供了实验依据。
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| 关 键 词： | 通痹灵总碱 小鼠 T淋巴细胞 CD69 |
| 文章编号： | 1007-8738(2003)01-93-02 |
| 修稿时间： | 2002年3月13日 |
Effect of overall alkali of Tongbiling on CD69 expression activated mouse T lymphocytes |
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| CHEN Ji-fan,CHEN Guang-xing,LI Xiao-juan,QUAN Shi-ming,ZENG Yao-ying,HU Ying-je,HUANG Ke-er,LIU Xiao-lingThe First Attached Hospital Guangzhou TCM University,Guangzhou ,China.Effect of overall alkali of Tongbiling on CD69 expression activated mouse T lymphocytes[J].Journal of Cellular and Molecular Immunology,2003,19(1):93-94. |
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| Authors: | CHEN Ji-fan CHEN Guang-xing LI Xiao-juan QUAN Shi-ming ZENG Yao-ying HU Ying-je HUANG Ke-er LIU Xiao-lingThe First Attached Hospital Guangzhou TCM University Guangzhou China |
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| Institution: | The First Attached Hospital, Guangzhou TCM University, Guangzhou 510405, China. guangxing_chen@hotmail.com |
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| Abstract: | AIM: To investigate the effect of overall alkali of Tongbiling (TBL) on CD69 expression on activated mouse T lymphocytes and its possible mechanism. METHODS: Phorbol 12, 13-dibutyrate( PDB) or concanavalin (ConA) were added successively into mouse lymphocytic culture with various concentration of overall alkali TBL. After 24 hours, CD69 expression rate on mouse T lymphocytes activated with PDB or ConA was analyzed by flow cytometry. RESULTS: Overall alkali TBL could significantly down-regulate CD69 expression in a dose-dependent maner. CONCLUSION; Overall alkali TBL can significantly inhibit CD69 expression on activated mouse T lymphocytes. This study provided an experimental basis for application of overall alkali TBL to treatment of rheumatoid arthritis. |
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| Keywords: | overall alkali of Tongbiling T lymphocytes activation CD69 expression |
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