The PWM model of the immunoglobulin synthesis capacity of human peripheral blood lymphocytes. II. Modification of the immunofluorescent detection of cytoplasmic immunoglobulins (cIg+)

To detect intracytoplasmic antibodies after PWM-stimulation of peripheral mononuclear cells we used class specific antisera against IgG, A and M and ascertained the whole number of cIg+-cells by addition. The number of cIg+-cells after culturing 7 days without mitogen was below 1%, with PWM-stimulation 14,7% +/- 8,5 (n = 29, blood donors). We discussed the requirements for the culture conditions and for the immunofluorescence detection.