| hTERT启动子调控的TRAIL基因表达载体的构建及其对肝癌细胞增殖的抑制作用 |
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| 引用本文： | 李金华,刘扬,王宏芳,王志成,吴嘉慧,龚守良,李娟. hTERT启动子调控的TRAIL基因表达载体的构建及其对肝癌细胞增殖的抑制作用[J]. 吉林大学学报(医学版), 2010, 36(5): 825-831 |
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| 作者姓名： | 李金华 刘扬 王宏芳 王志成 吴嘉慧 龚守良 李娟 |
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| 作者单位： | 吉林大学公共卫生学院,卫生部放射生物学重点实验室,吉林,长春,130021;吉林大学公共卫生学院卫生检验教研室,吉林,长春,130021;吉林大学公共卫生学院,卫生部放射生物学重点实验室,吉林,长春,130021;吉林大学公共卫生学院卫生检验教研室,吉林,长春,130021 |
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| 基金项目： | 国家自然科学基金资助课题(30870747,30970681) |
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| 摘 要： | 目的：构建hTERT启动子调控的肿瘤坏死因子相关的凋亡诱导配体(TRAIL)基因表达载体，探讨其对肝癌细胞SMMC7721和肝细胞HL7702增殖的抑制作用。方法：采用分子生物学方法构建重组质粒pshuttle-TRAIL和pshuttle-hTERT-TRAIL，经PCR和酶切鉴定正确后，通过脂质体转染SMMC7721和HL7702细胞，同时转染pcDNA3.1+-GFP并用荧光显微镜检测转染效率。MTT法检测重组质粒对细胞增殖变化的影响。结果：成功构建重组质粒pshuttle-TRAIL和pshuttle-hTERT-TRAIL，荧光显微镜检测结果显示：质粒与脂质体的比例为1∶3时细胞转染效率最高。pshuttle-hTERT-TRAIL组SMMC7721细胞增殖抑制率从16 h开始明显增加，与对照组比较差异有显著性（P<0.001），且从24 h开始与pshuttle-TRAIL组比较细胞增殖抑制率明显增加（P<0.05）；但重组质粒对HL7702细胞增殖抑制率无影响。结论： hTERT启动子调控的TRAIL可明显抑制肝癌细胞的增殖，而对人肝细胞的增殖无影响，其作用效果明显优于TRAIL单基因治疗。
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| 关 键 词： | 肿瘤坏死因子相关的凋亡诱导配体 hTERT启动子 肿瘤靶向治疗 细胞增殖 |
| 收稿时间： | 2010-05-12 |
Construction of hTERT promoter-driven TRAIL expression vector and its inhibitory effects on hepatoma cell proliferation |
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| LI Jin-hua,LIU Yang,WANG Hong-fang,WANG Zhi-cheng,WU Jia-hui,GONG Shou-liang,LI Juan. Construction of hTERT promoter-driven TRAIL expression vector and its inhibitory effects on hepatoma cell proliferation[J]. Journal of Jilin University: Med Ed, 2010, 36(5): 825-831 |
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| Authors: | LI Jin-hua LIU Yang WANG Hong-fang WANG Zhi-cheng WU Jia-hui GONG Shou-liang LI Juan |
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| Affiliation: | Key Laboratory of Radiobiology|Ministry of Health|School of Public Health|Jilin University,Changchun 130021,China;2. Department of Health Laboratory,School of Public Health,Jilin University,Changchun 130021,China |
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| Abstract: | Objective To construct the hTERT promoter-driven TRAIL expression vector and explore its inhibitory effects on the proliferation of hepatoma SMMC7721cells and HL7702hepatocytes.Methods The recombinant plasmids pshuttle-TRAIL and pshuttle-hTERT-TRAIL were constructed with molecular biological methods.They were confirmed correctly by endonucleases digestion and PCR identification,and transfected into SMMC7721and HL7702cells through liposome,meanwhile,the pcDNA3.1+-GFP was transfected to detect the transfectio... |
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| Keywords: | TNF-related apoptosis inducing ligand hTERT promoter tumor targeting therapy cell proliferation |
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