千里光对金黄色葡萄球菌和大肠埃希菌生物合成的影响

目的 应用血清药理学方法研究中药千里光对金黄色葡萄球菌和大肠埃希菌生物合成的影响。方法 从千里光中分离出黄酮类化合物，然后通过给小鼠灌胃制备千里光水浸液的含药血清和黄酮类化合物的含药血清。用同位素前体参入试验测定各含药血清对3H-TdR、3H-UdR、3H-亮氨酸和3H-葡糖胺等4种同位素前体参入金黄色葡萄球菌和大肠埃希菌的抑制率。同时设立生理盐水血清作为阴性对照。结果 与阴性对照血清相比，2种含药血清对3H-TdR、3H-UdR、3H-亮氨酸和3H-葡糖胺等4种同位素前体参入金黄色葡萄球菌和大肠埃希菌均有明显的抑制作用(P＜0.01)，且2种含药血清对受试菌的同位素前体参入抑制作用相当(P＞0.05)。结论 千里光的抗菌作用机制可能与通过抑制细菌的DNA、RNA、蛋白质和肽聚糖的合成有关，其作用的有效成份可能是黄酮类化合物。

Effect of Senecio Scandens Buch-Ham on the biosynthesis of Staphylococcus aureus and Escherichia coi

To explore the effect of Senecio Scandens Buch-Ham(SSB) on the biosynthesis of Staphylococcus aureus and Escherichia coil by serum pharmacological method. Method Flavonoid compound was isolated from SSB, and then the serum contained water decoct agent of SSB or flavonoid compound were prepared with mice. By means of incorporation experiment of isotope-labelled precursor, the incorporation inhibitation rates of 3H-TdR, 3H-UdR, 3H-leucine and 3H-glucosamine which would be incorporated into S.aureus and E.coil were measured after treatment with the serum of SSB water decoct agent or flavonoid, respectively. Meanwhile, the serum contained normal saline was set as negative control. Results Compared with negative control serum, both drug containing serum could obviously inhibit incorporation of 3H-TdR , 3H-UdR, 3H-leucine and 3H-glucosamine into S. aureus and E.coil (P＜0.01), but the inhibitation effect of both serum on bacterium were no significant difference (P＞0.05). Conclusion The antibacterial mechanism of SSB may be involved with the inhibition of DNA, RNA, protein and peptidoglycan synthesis, its effective ingredients may be flavonoid compound.